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Amphotericin B

Catalog No. T1067   CAS 1397-89-3
Synonyms: NSC 527017

Amphotericin B (NSC-527017) is a polyene antifungal agent with broad-spectrum activity against many fungal species. Amphotericin B irreversibly binds to ergosterol and disrupts the integrity of cell membranes, resulting in antifungal activity.

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Amphotericin B Chemical Structure
Amphotericin B, CAS 1397-89-3
Pack Size Availability Price/USD Quantity
50 mg In stock $ 33.00
100 mg In stock $ 46.00
500 mg In stock $ 76.00
1 g In stock $ 98.00
1 mL * 10 mM (in DMSO) In stock $ 32.00
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Potency: 847ug/mg
Potency: >750ug /mg
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Amphotericin B (NSC-527017) is a polyene antifungal agent with broad-spectrum activity against many fungal species. Amphotericin B irreversibly binds to ergosterol and disrupts the integrity of cell membranes, resulting in antifungal activity.
In vitro METHODS: Human normal colon epithelial cells CCD 841 CoTr and human colorectal cancer cells HT-29 were treated with Amphotericin B (0.05-25 µg/mL) for 24 h. Cell viability was measured by Neutral Red method.
RESULTS: Higher concentrations of Amphotericin B were toxic to CCD 841 CoTr and HT-29 cells, with IC50 values of 8.7 µg/mL and 21.2 µg/mL, respectively. [1]
METHODS: Candida albicans were treated with Amphotericin B (100 µM) for 4-16 min and imaged using the FLIM technique.
RESULTS: Amphotericin B preferentially binds to the cell wall and does not efficiently cross the barrier covering the cell membrane.Amphotericin B can more readily pass through the cell wall barrier of young cells during the emergence stage. [2]
In vivo METHODS: To assay antifungal activity in vivo, Amphotericin B (0.25-4 mg/kg) was administered as a single intraperitoneal injection to C. albicans K-1-infected ICR/Swiss mice.
RESULTS: Only the highest single dose of Amphotericin B treatment significantly reduced the number of colonies compared to the number of colonies at the start of treatment. [3]
Kinase Assay THP-1 and HEK293 cells are transiently transfected using DEAE-dextran and Polyfect reagent, respectively. Plasmids transfected contain genes coding for the NF-κB-dependent pELAM-luc luciferase reporter, TLR2, TLR4, CD14, and MD2. Cells (5×105 THP-1 or 1×105 HEK293) are added to 12-well plates, washed after 18 h, and stimulated for 5 h. Cells are then lysed with reporter lysis buffer as directed, and lysates are analyzed for luminescence using Promega luciferase substrate and a Monolight 3010 luminometer.
Cell Research Amphotericin B is dissolved in DMSO. The kinetics of cell death induced by AmB against Leishmania promastigotes is followed by using fluorometry with the DNA-binding compound ethidium bromide (EB). Fluorescence measurements are performed on a SPEX Fluorolog II spectrophotometer at 365-580 nm excitation-emission wavelengths. Promastigotes at a final concentration of 25×106 cells/mL are incubated for 5 min with gentle stirring in the fluorescence cuvette with 2 mL of different buffered solutions but always containing 10 mM glucose and EB (50 mM). After signal stabilization is achieved, AmB is added and dissolved in dimethylsulfoxide. Maximal EB incorporation is always obtained by adding digitonin (50 mg/mL). All solutions used are buffered with 75 mM TRIS (pH 4 7.6) and contain 150 mM NaCl (BNa+), 150 mM KCl (BK+), 150 mM choline chloride, and 100 mM sucrose, 100 mM NaCl. The osmolarity of all solutions is always adjusted to 390±5 mOsm using an advanced instrument SW2 osmometer.
Synonyms NSC 527017
Molecular Weight 924.08
Formula C47H73NO17
CAS No. 1397-89-3

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 50 mg/mL (54.11 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

TargetMolReferences and Literature

1. Grela E, et al. Imaging of human cells exposed to an antifungal antibiotic amphotericin B reveals the mechanisms associated with the drug toxicity and cell defence. Sci Rep. 2018 Sep 14;8(1):14067. 2. Grela E, et al. Modes of the antibiotic activity of amphotericin B against Candida albicans. Sci Rep. 2019 Nov 19;9(1):17029. 3. Andes D, Pet al. Pharmacodynamics of amphotericin B in a neutropenic-mouse disseminated-candidiasis model. Antimicrob Agents Chemother. 2001 Mar;45(3):922-6. 4. Demaimay R, et al. J Gen Virol, 1994, 75 ( Pt 9), 2499-2503. 5. Adams ML, et al. Biomacromolecules, 2003, 4(3), 750-757.

Related compound libraries

This product is contained In the following compound libraries:
Microbial Natural Product Library Anti-Infection Compound Library Antibiotics Library Anti-infective Natural Product Library Covalent Inhibitor Library Anti-Fungal Compound Library Antiparasitic Natural Product Library Bioactive Compounds Library Max Natural Product Library for HTS NO PAINS Compound Library

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Keywords

Amphotericin B 1397-89-3 Microbiology/Virology Antifungal Antibiotic Fungal NSC527017 NSC-527017 Inhibitor NSC 527017 inhibit inhibitor

 

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