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α-Linolenic acid

Name: α-Linolenic acid
Brand: TargetMol
SKU: T3P2904
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3P2904Cas No. 463-40-1

Alias Linolenic acid, Alpha-Linolenic Acid

α-Linolenic Acid (ALA) is an essential fatty acid that cannot be synthesized by the human body and is obtained by isolating it from seed oils. α-Linolenic acid has been shown to improve memory, inhibit thrombosis, and lower blood lipids.

α-Linolenic acid

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Purity: 99.81%

Catalog No. T3P2904Alias Linolenic acid, Alpha-Linolenic AcidCas No. 463-40-1

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
100 mg	$33	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.81%

Color:Transparent

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COA

Product Introduction

Bioactivity

Description	α-Linolenic Acid (ALA) is an essential fatty acid that cannot be synthesized by the human body and is obtained by isolating it from seed oils. α-Linolenic acid has been shown to improve memory, inhibit thrombosis, and lower blood lipids.
In vitro	METHODS: Osteosarcoma cells MG63, 143B and U2OS were treated with α-Linolenic Acid (10-200 µM) for 24 h. Cell viability was determined by MTT assay. RESULTS: α-Linolenic Acid showed a highly inhibitory effect on cell proliferation in a dose-dependent manner, with IC50 values of 51.69±0.14 µM in MG63 cells, 56.93±0.23 µM in 143B cells and 49.8±0.50 µM in U2OS cells.[1] METHODS: Breast cancer cells MDA-MB-231 and MCF-7 were treated with α-Linolenic Acid (25-100 µM) for 24 h. Apoptosis was detected by flow cytometer. RESULTS: The percentage of apoptotic cells in the early (from 2.9% to 23.5% and from 5.5% to 16.7%, respectively) and late (from 0.6% to 3.4% and from 2.0% to 13.8%, respectively) apoptotic stages was significantly increased in MDA-MB-231 and MCF-7 cells treated with 100 µM α-Linolenic Acid. percentage significantly increased, which was significantly higher compared to untreated control cells. [2]
In vivo	METHODS: To study the therapeutic effect on colitis in mice, α-Linolenic Acid (150-300 mg/kg, dispersed in 0.25% Tween-20) was administered by gavage to HFHSD-fed BABL/c mice once daily for nine weeks. At the end of nine weeks, experimental colitis was induced by intracolonic injection of TNBS. RESULTS: Low α-Linolenic Acid administration significantly ameliorated TNBS-induced clinical manifestations, body weight loss, spleen weight loss, and histologic damage. In contrast, high α-Linolenic Acid administration did not improve colitis and even exacerbated symptoms.HFHSD exacerbated TNBS-induced colitis via the Th1/Th17 pathway. Low α-Linolenic Acid showed a protective effect against TNBS-induced colitis via the Th1/Th2/Th17 pathway. [3]
Synonyms	Linolenic acid, Alpha-Linolenic Acid

Chemical Properties

Molecular Weight	278.43
Formula	C₁₈H₃₀O₂
Cas No.	463-40-1
Smiles	CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O
Relative Density.	0.914 g/cm3 at 25℃ (lit.)

Storage & Solubility Information

Storage

store at low temperature,store under nitrogen | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 100 mg/mL (359.16 mM), Sonication is recommended.

DMSO: 100 mg/mL (359.16 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 10 mg/mL (35.92 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.5916 mL	17.9578 mL	35.9157 mL	179.5784 mL
5 mM	0.7183 mL	3.5916 mL	7.1831 mL	35.9157 mL
10 mM	0.3592 mL	1.7958 mL	3.5916 mL	17.9578 mL
20 mM	0.1796 mL	0.8979 mL	1.7958 mL	8.9789 mL
50 mM	0.0718 mL	0.3592 mL	0.7183 mL	3.5916 mL
100 mM	0.0359 mL	0.1796 mL	0.3592 mL	1.7958 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc