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ZCL278

Name: ZCL278
Brand: TargetMol
SKU: T1855
Price: 46 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1855Cas No. 587841-73-4

Alias ZCL 278

ZCL278 is a selective Cdc42 GTPase inhibitor.

ZCL278

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Purity: 99.72%

Catalog No. T1855Alias ZCL 278Cas No. 587841-73-4

ZCL278 is a selective Cdc42 GTPase inhibitor.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$46	In Stock	In Stock
10 mg	$72	In Stock	In Stock
25 mg	$138	In Stock	In Stock
50 mg	$233	In Stock	In Stock
100 mg	$327	In Stock	In Stock
200 mg	$523	In Stock	In Stock
500 mg	$848	-	In Stock
1 mL x 10 mM (in DMSO)	$72	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.72%

Color:White

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	ZCL278 is a selective Cdc42 GTPase inhibitor.
Targets&IC50	GTPase CDC42:11.4 μM(Kd)
In vivo	ZCL278 reduces the JUNV RNA load in the spleen by over 33-fold, rendering JUNV RNA undetectable in 5 of 8 mice. These results mirror those observed in Gabapentin-treated mice, demonstrating ZCL278's ability to abrogate JUNV replication [2].
Kinase Assay	Lyophilized Cdc42 protein is reconstituted to 5 mg/mL in a buffer consisting of 50 mM Tris, 0.5 mM MgCl2, 50 mM NaCl, 3% (wt/vol) sucrose, and 0.6% dextran. The stock solution is then diluted to 1 μM in 5 mM phosphate buffer, pH 7.4. Into a quartz cuvettete containing Cdc42 solution, aliquots of ZCL278 are added and incubated for 5 min before each fluorescent measurement. The excitation wavelength is 275 nm, and the fluorescence of tryptophan at 350 nm is measured after each addition. The titration curve is fitted using the equimolar specific binding model in GraphPad, and the Kd is calculated[1].
Cell Research	ZCL278 is prepared in DMSO and stored, and then diluted with appropriate medium before use[1]. To determine cell viability, PC-3 cells are incubated for 24 h with or without the Cdc42 activator, ZCL278, or NSC23766. By using the trypan blue dye exclusion method, the numbers of live and dead cells are obtained with a Countess Automated Cell Counter. P values are assigned in each experiment, and any null hypothesis with probability level <95% is rejected[1].
Synonyms	ZCL 278

Chemical Properties

Molecular Weight	584.89
Formula	C₂₁H₁₉BrClN₅O₄S₂
Cas No.	587841-73-4
Smiles	Cc1cc(C)nc(NS(=O)(=O)c2ccc(NC(=S)NC(=O)COc3ccc(Br)cc3Cl)cc2)n1
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (68.39 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (3.42 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7097 mL	8.5486 mL	17.0972 mL	85.4862 mL
5 mM	0.3419 mL	1.7097 mL	3.4194 mL	17.0972 mL
10 mM	0.1710 mL	0.8549 mL	1.7097 mL	8.5486 mL
20 mM	0.0855 mL	0.4274 mL	0.8549 mL	4.2743 mL
50 mM	0.0342 mL	0.1710 mL	0.3419 mL	1.7097 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc