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Z-FA-FMK

Name: Z-FA-FMK
Brand: TargetMol
SKU: T6738
Price: 61 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T6738Cas No. 197855-65-5

Z-FA-FMK can irreversibly inhibit cysteine protease and also inhibit effector caspases.

Z-FA-FMK

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Purity: 99.68%

Catalog No. T6738Cas No. 197855-65-5

Z-FA-FMK can irreversibly inhibit cysteine protease and also inhibit effector caspases.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$61	In Stock	In Stock
5 mg	$198	In Stock	In Stock
10 mg	$297	In Stock	In Stock
25 mg	$479	In Stock	In Stock
50 mg	$653	In Stock	In Stock
100 mg	$929	-	In Stock
1 mL x 10 mM (in DMSO)	$178	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.68%

Color:White

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Product Introduction

Bioactivity

Description	Z-FA-FMK can irreversibly inhibit cysteine protease and also inhibit effector caspases.
Targets&IC50	3CLpro (SARS Protease):25.7 μM (Ki)
In vitro	Z-FA-FMK inhibits the development of Ras-induced carcinogenic tumors and respiratory viral infections in the cardiac tissue of mice with severe combined immunodeficiency (SCID). In a mouse model of intranasal pneumococcal infection, Z-FA-FMK significantly promotes the growth of Streptococcus pneumoniae in both the lungs and the bloodstream.
In vivo	Z-FA-FMK effectively inhibits T-cell proliferation induced by interleukin-2 (IL-2) and mitogens in vitro. It also prevents the degradation of fibrillar collagen through its actions on fibroblasts and osteoclasts. Furthermore, Z-FA-FMK suppresses the expression of NF-κB-dependent genes in macrophages, thereby inhibiting the production of cytokines induced by lipopolysaccharides.
Cell Research	T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester. (Only for Reference)

Chemical Properties

Molecular Weight	386.42
Formula	C₂₁H₂₃FN₂O₄
Cas No.	197855-65-5
Smiles	CC(NC(=O)[C@H](Cc1ccccc1)NC(=O)OCc1ccccc1)C(=O)CF
Relative Density.	no data available
Sequence	Cbz-Phe-DL-Ala-CH2F
Sequence Short	FA

Storage & Solubility Information

Storage

store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 71 mg/mL (183.74 mM), Sonication is recommended.

Ethanol: 32 mg/mL (82.81 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.18 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5879 mL	12.9393 mL	25.8786 mL	129.3929 mL
5 mM	0.5176 mL	2.5879 mL	5.1757 mL	25.8786 mL
10 mM	0.2588 mL	1.2939 mL	2.5879 mL	12.9393 mL
20 mM	0.1294 mL	0.6470 mL	1.2939 mL	6.4696 mL
50 mM	0.0518 mL	0.2588 mL	0.5176 mL	2.5879 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0259 mL	0.1294 mL	0.2588 mL	1.2939 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc