Anti-Cathepsin B Polyclonal Antibody is a Rabbit antibody targeting Cathepsin B. Anti-Cathepsin B Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat

1. Tissue/cell: Human esophageal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Cathepsin B Polyclonal Antibody, Unconjugated (TMAB-00297) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Spleen (Mouse) Lysate at 40 μg
Primary: Anti-Cathepsin B (TMAB-00297) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46/28 kDa
Observed band size: 30 kDa
3. Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-Cathepsin B antibody (TMAB-00297), Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit Igg (orange),used under the same conditions); Secondary Antibody: Goat anti-rabbit IgG-Pe (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min). Antibody (TMAB-00297, 5 μg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of TMAB-00297 at 1/200 dilution for 30 min on ice.

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1µg/Test

Polyclonal

KLH conjugated synthetic peptide: human Cathepsin B heavy chain

Human

Cathespins,Cell adhesion,Extracellular matrix,Adhesion molecules ELISA kits,Membrane Proteins,Other Enzymes