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Rutaecarpine

Name: Rutaecarpine
Brand: TargetMol
SKU: T2972
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2972Cas No. 84-26-4

Alias Rutecarpine, Rutacarpine, Rhetine

Rutaecarpine (Rhetine) is an inhibitor of COX-2 with IC50 of 0.28 μM.

Rutaecarpine

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Purity: 99.2%

Catalog No. T2972Alias Rutecarpine, Rutacarpine, RhetineCas No. 84-26-4

Rutaecarpine (Rhetine) is an inhibitor of COX-2 with IC50 of 0.28 μM.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$33	In Stock	In Stock
10 mg	$52	In Stock	In Stock
25 mg	$79	In Stock	In Stock
50 mg	$108	In Stock	In Stock
100 mg	$163	In Stock	In Stock
500 mg	$397	-	In Stock
1 mL x 10 mM (in DMSO)	$50	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.2%

Color:Yellow

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	Rutaecarpine (Rhetine) is an inhibitor of COX-2 with IC50 of 0.28 μM.
Targets&IC50	COX-1:8.7 μM (in BMMC), COX-2:0.28 μM
In vitro	Rutaecarpine is an indolopyridoquinazolinone alkaloid isolated from Evodia rutaecarpa and related herbs, which has shown a variety of intriguing biological properties such as anti-thrombotic, anticancer, anti-inflammatory and analgesic, anti-obesity and thermoregulatory, vasorelaxing activity, as well as effects on the cardiovascular and endocrine systems. [1]
Cell Research	Rutaecarpine is dissolved in DMSO and diluted with appropriate medium before use. COX-1 and COX-2 cDNA-transfected HEK293 cells are prepared. For measuring inhibitory activity on COX-1 and COX-2 by rutaecarpine, cells in 1 mL of culture medium are seeded into each well of 24-well. After culture for 4 days, the supernatants are removed and 250 mL of fresh medium is added to the cells with or without rutaecarpine. After preincubation for 5 h at 37°C, the cells are further incubated at 37°C for 30 min with 50 mM arachidonic acid. All reactions are stopped by centrifugation at 120 g at 4°C for 5 min. Concentrations of PGE2 in the supernatant are measured[1].
Synonyms	Rutecarpine, Rutacarpine, Rhetine

Chemical Properties

Molecular Weight	287.32
Formula	C₁₈H₁₃N₃O
Cas No.	84-26-4
Smiles	O=C1N2CCC3=C(NC4=CC=CC=C34)C2=NC2=CC=CC=C12
Relative Density.	1.45 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 0.3 mg/mL (1.04 mM)

DMSO: 50 mg/mL (174.02 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

PBS: < 1 mg/mL (insoluble)

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.4804 mL	17.4022 mL	34.8044 mL	174.0220 mL

DMSO

	1mg	5mg	10mg	50mg
5 mM	0.6961 mL	3.4804 mL	6.9609 mL	34.8044 mL
10 mM	0.3480 mL	1.7402 mL	3.4804 mL	17.4022 mL
20 mM	0.1740 mL	0.8701 mL	1.7402 mL	8.7011 mL
50 mM	0.0696 mL	0.3480 mL	0.6961 mL	3.4804 mL
100 mM	0.0348 mL	0.1740 mL	0.3480 mL	1.7402 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc