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Phenylbutazone

Name: Phenylbutazone
Brand: TargetMol
SKU: T1432
Price: 38 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1432Cas No. 50-33-9

Alias Butazolidine

Phenylbutazone (Butazolidine) is a butyl-diphenyl-pyrazolidinedione that has anti-inflammatory, antipyretic, and analgesic activities. It has been used in ankylosing spondylitis, rheumatoid arthritis, and reactive arthritis.

Phenylbutazone

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Purity: 99.93%

Catalog No. T1432Alias ButazolidineCas No. 50-33-9

Pack Size	Price	USA Warehouse	Global Warehouse
1 g	$38	-	In Stock
5 g	$98	-	In Stock
1 mL x 10 mM (in DMSO)	$53	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.93%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Phenylbutazone AI Summary

Phenylbutazone exhibits significant anti-inflammatory activity through multiple mechanisms, including the inhibition of carrageenan-induced paw edema in rats with varying levels of dose-dependent inhibition. The compound demonstrates inhibitory activity against canine COX-2 with an IC50 of 3790.0 nM and oval tissue PGH synthase with an IC50 of 10000.0 nM, as well as binding affinity to cytochrome P450 2C9 with a Ki of 19000.0 nM. The anti-inflammatory effects are evident in both the carrageenan-induced and adjuvant-induced models, with ED50 values ranging from 10.0 mg/kg to 37.5 mg/kg depending on the specific assay. Besides its anti-inflammatory properties, Phenylbutazone has analgesic effects, as shown by reduced writhing in the acetic acid test and increased reaction time in the hot plate test. However, it also poses potential risks, including ulcerogenic effects at higher doses and moderate acute toxicity, with LD50 values reported around 300 mg/kg in mice. The compound possesses a partition coefficient (logP) of 3.04, pKa of 4.4, and notable binding affinity to human serum albumin (log B/F value of 1.619). Additionally, it demonstrates moderate to high activity in displacing other compounds from human serum albumin binding sites. Moreover, Phenylbutazone shows varying inhibitory effects on human liver enzymes UGT1A6 and UGT1A9 and on transporter systems, indicating possible interactions affecting drug metabolism and transport. Despite moderate hepatotoxic risks and demonstrated ulcerogenic activity, the compound's multi-target action profile underscores its potential therapeutic applications, albeit with necessary considerations for dosage and side effects..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Phenylbutazone (Butazolidine) is a butyl-diphenyl-pyrazolidinedione that has anti-inflammatory, antipyretic, and analgesic activities. It has been used in ankylosing spondylitis, rheumatoid arthritis, and reactive arthritis.
Synonyms	Butazolidine

Chemical Properties

Molecular Weight	308.37
Formula	C₁₉H₂₀N₂O₂
Cas No.	50-33-9
Smiles	CCCCC1C(=O)N(N(C1=O)C1=CC=CC=C1)C1=CC=CC=C1
Relative Density.	1.173g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (178.36 mM), Sonication is recommended.

Ethanol: 58 mg/mL (188.09 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (6.49 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	3.2429 mL	16.2143 mL	32.4286 mL	162.1429 mL
5 mM	0.6486 mL	3.2429 mL	6.4857 mL	32.4286 mL
10 mM	0.3243 mL	1.6214 mL	3.2429 mL	16.2143 mL
20 mM	0.1621 mL	0.8107 mL	1.6214 mL	8.1071 mL
50 mM	0.0649 mL	0.3243 mL	0.6486 mL	3.2429 mL
100 mM	0.0324 mL	0.1621 mL	0.3243 mL	1.6214 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc