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PF-543 hydrochloride

Name: PF-543 hydrochloride
Brand: TargetMol
SKU: T8840
Price: 33 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T8840Cas No. 1706522-79-3

Alias PF-543 HCL

PF-543 hydrochloride (PF-543) inhibits SphK1 with a K(i) of 3.6 nM, is sphingosine-competitive and is more than 100-fold selective for SphK1 over the SphK2 isoform.

PF-543 hydrochloride

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Purity: 99.93%

Catalog No. T8840Alias PF-543 HCLCas No. 1706522-79-3

PF-543 hydrochloride (PF-543) inhibits SphK1 with a K(i) of 3.6 nM, is sphingosine-competitive and is more than 100-fold selective for SphK1 over the SphK2 isoform.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$33	In Stock	In Stock
5 mg	$70	In Stock	In Stock
10 mg	$137	In Stock	In Stock
25 mg	$248	In Stock	In Stock
50 mg	$335	In Stock	In Stock
100 mg	$513	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$105	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.93%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	PF-543 hydrochloride (PF-543) inhibits SphK1 with a K(i) of 3.6 nM, is sphingosine-competitive and is more than 100-fold selective for SphK1 over the SphK2 isoform.
Targets&IC50	S1P1:26.7 nM (IC50), SPHK1:2 nM (IC50), SPHK1:3.6 nM (Ki)
In vitro	SphK1 inhibition by PF-543 causes a dose-dependent depletion of the intracellular level of S1P with EC50 concentration of 8.4 nM and a concomitant elevation of the intracellular level of sphingosine in 1483 cells. The level of endogenous S1P in 1483 cells after a 1 h treatment with 200 nM PF-543 is decreased 10-fold, producing a proportional increase in the level of sphingosine.
In vivo	PF543 was found to be a potent inhibitor (IC50 = 26.7 nM) capable of blocking >90% of C17-S1P formation in the blood.
Synonyms	PF-543 HCL

Chemical Properties

Molecular Weight	502.1
Formula	C₂₇H₃₂ClNO₄S
Cas No.	1706522-79-3
Smiles	Cl.Cc1cc(CS(=O)(=O)c2ccccc2)cc(OCc2ccc(CN3CCC[C@@H]3CO)cc2)c1
Relative Density.	no data available

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (99.58 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.9916 mL	9.9582 mL	19.9164 mL	99.5818 mL
5 mM	0.3983 mL	1.9916 mL	3.9833 mL	19.9164 mL
10 mM	0.1992 mL	0.9958 mL	1.9916 mL	9.9582 mL
20 mM	0.0996 mL	0.4979 mL	0.9958 mL	4.9791 mL
50 mM	0.0398 mL	0.1992 mL	0.3983 mL	1.9916 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc