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N-Ethylmaleimide

Name: N-Ethylmaleimide
Brand: TargetMol
SKU: T3088
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3088Cas No. 128-53-0

Alias NEM, Ethylmaleimide, 1-Ethyl-1H-pyrrole-2,5-dione

N-Ethylmaleimide (NEM) is a reagent for alkylation of free sulfhydryl groups, a cysteine protease inhibitor used in experimental biochemistry. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor that specifically inhibits phosphate transport in mitochondria.

N-Ethylmaleimide

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Purity: 99.50%

Catalog No. T3088Alias NEM, Ethylmaleimide, 1-Ethyl-1H-pyrrole-2,5-dioneCas No. 128-53-0

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$39	In Stock	In Stock
25 mg	$62	In Stock	In Stock
100 mg	$82	In Stock	In Stock
200 mg	$98	In Stock	In Stock
500 mg	$158	In Stock	In Stock
1 g	$236	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$43	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.50%

Color:White

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	N-Ethylmaleimide (NEM) is a reagent for alkylation of free sulfhydryl groups, a cysteine protease inhibitor used in experimental biochemistry. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor that specifically inhibits phosphate transport in mitochondria.
Targets&IC50	prolyl endopeptidase:6.3 μM, COS-7 cells:43.7 μM (EC50), KB-3-1 cells:0.03 mM, SF-268 cells:6.5 μg/mL (GL50), KB-V1 cells:0.016 mM, MCF-7 cells:9.9 μg/mL (GL50), NCI-H4 cells:6.7 μg/mL (GL50)
In vitro	METHODS: Growth-arrested VSMC cells were treated with N-Ethylmaleimide (20 µM) and PDGF-BB (20 ng/mL) for 5 min - 2 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: N-Ethylmaleimide inhibited PDGF BB-stimulated Akt phosphorylation. [1] METHODS: Jurkat T cells were treated with N-Ethylmaleimide (3-100 µM) for 1-20 min, and target protein expression levels were detected by immunoblots. RESULTS: Treatment of intact cells with 50-100 µM N-Ethylmaleimide for 5-10 min resulted in a significant increase in tyrosine residue phosphorylation. [2]
In vivo	METHODS: To examine the potential enhancement of sleep properties induced by alprazolam (Alp), N-Ethylmaleimide (1 mg/kg, 1% CMC-Na) and Alp (1.84 mg/kg, 1% CMC-Na) were administered by gavage to C57BL/6J mice. RESULTS: Combined administration of Alp and NEM significantly reduced sleep latency and prolonged sleep duration compared with Alp alone. This effect was characterized by a significant increase in REM duration. [3]
Synonyms	NEM, Ethylmaleimide, 1-Ethyl-1H-pyrrole-2,5-dione

Chemical Properties

Molecular Weight	125.13
Formula	C₆H₇NO₂
Cas No.	128-53-0
Smiles	C(C)N1C(=O)C=CC1=O
Relative Density.	1.207 g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 237.5 mg/mL (1898.03 mM), Sonication is recommended.

H2O: 50 mg/mL (399.58 mM), Sonication and heating are recommended.

In Vivo Formulation

5% DMSO+95% Saline: 1.2 mg/mL (9.59 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	7.9917 mL	39.9584 mL	79.9169 mL	399.5844 mL
5 mM	1.5983 mL	7.9917 mL	15.9834 mL	79.9169 mL
10 mM	0.7992 mL	3.9958 mL	7.9917 mL	39.9584 mL
20 mM	0.3996 mL	1.9979 mL	3.9958 mL	19.9792 mL
50 mM	0.1598 mL	0.7992 mL	1.5983 mL	7.9917 mL
100 mM	0.0799 mL	0.3996 mL	0.7992 mL	3.9958 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc