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IOWH-032

Name: IOWH-032
Brand: TargetMol
SKU: T2486
Price: 40 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2486Cas No. 1191252-49-9

Alias IOWH032, IOWH 032

IOWH-032 (IOWH032) , a synthetic CFTR inhibitor, has been investigated for the treatment of cholera, diarrhea, and secretory diarrhea.

IOWH-032

🥰Excellent

Purity: 99%

Catalog No. T2486Alias IOWH032, IOWH 032Cas No. 1191252-49-9

IOWH-032 (IOWH032) , a synthetic CFTR inhibitor, has been investigated for the treatment of cholera, diarrhea, and secretory diarrhea.

Pack Size	Price	Availability
10 mg	$54	In Stock
25 mg	$94	In Stock
50 mg	$146	In Stock
100 mg	$228	In Stock
1 mL x 10 mM (in DMSO)	$40	In Stock

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Purity:99%

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Product Introduction

Bioactivity

Description	IOWH-032 (IOWH032) , a synthetic CFTR inhibitor, has been investigated for the treatment of cholera, diarrhea, and secretory diarrhea.
Targets&IC50	CFTR:1.01 μM
In vitro	In a closed-system mouse model, IOWH032 effectively inhibits the secretions induced by cholera toxin. Moreover, in a rat model with the cecum removed, IOWH032 (5 mg/kg) suppresses the excretion of waste.
In vivo	In T84-CFTR cells (IC50=6.87 μM), IOWH032 effectively inhibits CFTR activity.
Kinase Assay	Enzyme assays are performed using a homogeneous time-resolved fluorescence assay with recombinant epitope tagged kinase domains (JAK1, 837-1142; JAK2, 828-1132; JAK3, 718-1124; Tyk2, 873-1187) or full-length enzyme (cMET and Chk2) and peptide substrate. Each enzyme reaction is performed with or without test compound (11-point dilution), JAK, cMET, or Chk2 enzyme, 500 nM (100 nM for Chk2) peptide, ATP (at the Km specific for each kinase or 1 mM), and 2.0% DMSO in assay buffer. The calculated IC50 value is the compound concentration required for inhibition of 50% of the fluorescent signal. Additional kinase assays are performed at Cerep using standard conditions at 200 nM. Enzymes tested included: Abl, Akt1, AurA, AurB, CDC2, CDK2, CDK4, CHK2, c-kit, EGFR, EphB4, ERK1, ERK2, FLT-1, HER2, IGF1R, IKKα, IKKβ, JNK1, Lck, MEK1, p38α, p70S6K, PKA, PKCα, Src, and ZAP70[1].
Synonyms	IOWH032, IOWH 032

Chemical Properties

Molecular Weight	545.18
Formula	C₂₂H₁₅Br₂N₃O₄
Cas No.	1191252-49-9
Smiles	Oc1c(Br)cc(cc1Br)-c1noc(n1)C(=O)NCc1ccc(Oc2ccccc2)cc1
Relative Density.	1.670 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 93 mg/mL (170.59 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (6.05 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8343 mL	9.1713 mL	18.3426 mL	91.7128 mL
5 mM	0.3669 mL	1.8343 mL	3.6685 mL	18.3426 mL
10 mM	0.1834 mL	0.9171 mL	1.8343 mL	9.1713 mL
20 mM	0.0917 mL	0.4586 mL	0.9171 mL	4.5856 mL
50 mM	0.0367 mL	0.1834 mL	0.3669 mL	1.8343 mL
100 mM	0.0183 mL	0.0917 mL	0.1834 mL	0.9171 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc