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GW274150

Name: GW274150
Brand: TargetMol
SKU: T11518L
Price: 32 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11518LCas No. 210354-22-6

GW274150 is an orally active, selective and potent dual inhibitor of NADPH-dependent human nitric oxide synthase (iNOS) (Kd=40 nM) and rat inducible nitric oxide synthase (iNOS).GW274150 has a low activity against endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) in humans and rats. GW274150 showed protective effects in an inflammatory model of acute lung injury.

GW274150

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Purity: 99.90%

Catalog No. T11518LCas No. 210354-22-6

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$32	In Stock	In Stock
5 mg	$73	In Stock	In Stock
10 mg	$123	In Stock	In Stock
25 mg	$255	In Stock	In Stock
50 mg	$413	In Stock	In Stock
100 mg	$662	In Stock	-

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products. If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

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Purity:99.90%

Appearance:Solid

Color:Yellow

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	GW274150 is an orally active, selective and potent dual inhibitor of NADPH-dependent human nitric oxide synthase (iNOS) (Kd=40 nM) and rat inducible nitric oxide synthase (iNOS).GW274150 has a low activity against endothelial nitric oxide synthase (eNOS) and neuronal nitric oxide synthase (nNOS) in humans and rats. GW274150 showed protective effects in an inflammatory model of acute lung injury.
Targets&IC50	eNOS (human):544 μM(kd), nNOS (rat):252 μM(ED50), iNOS (human):2.19 μM(kd), iNOS:(kd)40 nM, iNOS:40 nM(kd), nNOS (human):177 μM(kd), iNOS (rat): 1.15 μM(ED50), iNOS (rat):1.15 μM(ED50)
In vitro	GW274150 inhibits intracellular iNOS in J774 cells with an IC50 of 0.2 μM and demonstrates >260-fold and 219-fold selectivity for eNOS and nNOS, respectively, in rat tissue, as well as >100-fold and >80-fold selectivity for human eNOS and nNOS, respectively.[1]
In vivo	GW274150 is a long-acting (half-life in rats is 5 hours) iNOS inhibitor, which can inhibit the LPS-mediated increase in plasma NO2- and NO3- levels 14 h after a single intraperitoneal administration (ED50=3 mg/kg ).[2] GW274150 (2.5, 5, and 10 mg/kg; i.p.; before carrageenan injection; SD rats) reduces the extent of carrageenan-induced lung injury in a dose-related manner. Pleural cavity edema formation and PMN infiltration were also significantly attenuated in a dose-related manner in rats.[2] GW274150 (30 mg/kg; oral; twice daily; 7 days; SD rats) resulted in significant neuroprotection, however, it showed bell-shaped neuroprotection in 6-OHDA rats with Parkinson's disease (PD) Ineffective at high doses in the model.[3]

Chemical Properties

Molecular Weight	219.3
Formula	C₈H₁₇N₃O₂S
Cas No.	210354-22-6
Smiles	C([C@@H](C(O)=O)N)CSCCNC(C)=N
Relative Density.	1.30 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: < 1 mg/mL (insoluble or slightly soluble)

H2O: 50 mg/mL (228 mM), Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	4.5600 mL	22.7998 mL	45.5996 mL	227.9982 mL
5 mM	0.9120 mL	4.5600 mL	9.1199 mL	45.5996 mL
10 mM	0.4560 mL	2.2800 mL	4.5600 mL	22.7998 mL
20 mM	0.2280 mL	1.1400 mL	2.2800 mL	11.3999 mL
50 mM	0.0912 mL	0.4560 mL	0.9120 mL	4.5600 mL
100 mM	0.0456 mL	0.2280 mL	0.4560 mL	2.2800 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc