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GW274150 phosphate

Name: GW274150 phosphate
Brand: TargetMol
SKU: T11518
Price: 99 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T11518Cas No. 438542-15-5

Alias GW 274150 phosphate

GW274150 phosphate is a selective, orally active iNOS inhibitor with an IC50 of 0.2 μM in J774 cells. It mitigates experimental renal ischaemia-reperfusion injury and exhibits analgesic effects in rat models of inflammatory and neuropathic pain.

GW274150 phosphate

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Purity: 98.02%

Catalog No. T11518Alias GW 274150 phosphateCas No. 438542-15-5

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
2 mg	$99	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:98.02%

Appearance:Solid

Color:White

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Product Introduction

Bioactivity

Description	GW274150 phosphate is a selective, orally active iNOS inhibitor with an IC50 of 0.2 μM in J774 cells. It mitigates experimental renal ischaemia-reperfusion injury and exhibits analgesic effects in rat models of inflammatory and neuropathic pain.
Targets&IC50	iNOS (human):40 nM (Kd), iNOS (human):2.19 μM, iNOS (rat):1.15 μM (ED50)
In vitro	GW274150 phosphate exhibits time-dependent inhibitory effects on iNOS in J774 cells, with an IC₅₀ of 0.2±0.04 μM [1]. GW274150 demonstrates significantly higher selectivity for iNOS derived from rat tissues compared to eNOS and nNOS, being 260-fold and 219-fold more selective, respectively. Similarly, GW274150 shows markedly superior selectivity for homo sapiens iNOS, exhibiting 100-fold and 80-fold greater potency compared to homo sapiens eNOS and homo sapiens nNOS, respectively [1].
In vivo	In the chondrus ocellatus gel-induced rat lung injury model, administration of GW274150 phosphate (2.5, 5, and 10 mg/kg, intraperitoneal injection) before chondrus ocellatus gel injection dose-dependently reduced the severity of lung injury and also significantly attenuated pleural cavity edema formation and polymorphonuclear leukocyte (PMNs) infiltration in a dose-related manner [2]. In the 6-OHDA-induced rat Parkinson's disease (PD) model, GW274150 phosphate administered orally (30 mg/kg, twice daily for 7 consecutive days) exhibited significant neuroprotective effects. However, it should be noted that the neuroprotective efficacy of this compound followed a bell-shaped curve, with its protective effects disappearing at higher doses [3].
Synonyms	GW 274150 phosphate

Chemical Properties

Molecular Weight	317.3
Formula	C₈H₂₀N₃O₆PS
Cas No.	438542-15-5
Smiles	P(=O)(O)(O)O.C([C@@H](C(O)=O)N)CSCCNC(C)=N
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 80 mg/mL (252.13 mM), Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	3.1516 mL	15.7580 mL	31.5159 mL	157.5796 mL
5 mM	0.6303 mL	3.1516 mL	6.3032 mL	31.5159 mL
10 mM	0.3152 mL	1.5758 mL	3.1516 mL	15.7580 mL
20 mM	0.1576 mL	0.7879 mL	1.5758 mL	7.8790 mL
50 mM	0.0630 mL	0.3152 mL	0.6303 mL	3.1516 mL
100 mM	0.0315 mL	0.1576 mL	0.3152 mL	1.5758 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc