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Cholesterol

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Catalog No. T0760Cas No. 57-88-5
Alias Cholesteryl alcohol, Cholesterin

Cholesterol is the primary sterol in mammals, accounting for approximately 20–25% of the plasma membrane structure. It plays a key role in regulating membrane fluidity, permeability, and protein function. As an endogenous agonist of estrogen-related receptor α (ERRα), cholesterol is widely involved in metabolic regulation and serves as a precursor for the synthesis of hormones and bile acids. It is commonly used in experimental models of hyperlipidemia.

Cholesterol
Other Forms of Cholesterol:

Cholesterol

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Purity: 99.94%
Catalog No. T0760Alias Cholesteryl alcohol, CholesterinCas No. 57-88-5
Cholesterol is the primary sterol in mammals, accounting for approximately 20–25% of the plasma membrane structure. It plays a key role in regulating membrane fluidity, permeability, and protein function. As an endogenous agonist of estrogen-related receptor α (ERRα), cholesterol is widely involved in metabolic regulation and serves as a precursor for the synthesis of hormones and bile acids. It is commonly used in experimental models of hyperlipidemia.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
500 mg$43In StockIn Stock
1 g$60In StockIn Stock
5 g$98-In Stock
10 g$123-In Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Purity:99.94%
Appearance:Solid
Color:White
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COA HNMR LCMS

Product Introduction

Bioactivity
Description
Cholesterol is the primary sterol in mammals, accounting for approximately 20–25% of the plasma membrane structure. It plays a key role in regulating membrane fluidity, permeability, and protein function. As an endogenous agonist of estrogen-related receptor α (ERRα), cholesterol is widely involved in metabolic regulation and serves as a precursor for the synthesis of hormones and bile acids. It is commonly used in experimental models of hyperlipidemia.
In vitro
METHODS: CD4+ T lymphocytes were incubated with 7-KC (17.5-70 µM) and Cholesterol-MβCD (17.5-70 µM) for 10 min, and T cell membrane order and disorder were assessed using di-4 ANEPPDHQ fluorescent dye.
RESULTS: After exposure to 7-KC, T cell membrane order was altered in a dose-dependent manner, with significant reconstitution of membrane order observed only in cells treated with 35 µM Cholesterol, while reconstitution with l7.5 µM Cholesterol induced minimal effects. [1]
METHODS: Human gastric cancer cells SNU601, SNU638 and SNU216 were treated with Cholesterol (25-100 µM) for 48 h and cell viability was measured using MTT Assay.
RESULTS: Cholesterol caused a dose-dependent decrease in cell viability in all three cell lines. [2]
In vivo
METHODS: To induce hypercholesterolemia, STD:ddY mice were fed a high cholesterol diet (1% cholesterol, 0.5% cholic acid, 0.5% olive oil and 93% standard mouse chow).
RESULTS: Cholesterol can be used to construct a mouse model of hypercholesterolemia. [3]
METHODS: To induce hyperlipidemia, CD-1 mice were fed a high cholesterol diet (2% cholesterol and 0.6% sodium deoxycholate).
RESULTS: Cholesterol can be used to construct a mouse model of hyperlipidemia. [4]
Disease Modeling Protocol
Hyperlipidemia model
  • Modeling Mechanism：

    A high-cholesterol diet can increase serum cholesterol, induce lipid deposition in the aorta, and activate the inflammatory response (increased aortic cytokines), making it suitable for research on the inflammatory mechanisms of hyperlipidemia.

  • Related Products：

    Cholesterol (T0760)

  • Modeling Method：

    Experimental Subject：Guinea pigs, Hartley strain, Male, 8 weeks old, Body weight 300–350 g

    Dosage and Administration Route：① Core modelling: - High-fat diet (0.5% cholesterol+5% butter); ② Control treatment: Control group administered cholesterol-free standard diet, all other conditions identical;

    Dosing Frequency and Duration Model：Ad libitum feeding, 10 weeks duration

  • Validation：

    1. Biochemical indicators: - Serum cholesterol: ≥5 mmol/L after 10 weeks of modeling (compared to 1.8 mmol/L in the control group); - Inflammatory factors: Serum TNF-α and IL-6 levels were significantly elevated; 2. Pathological indicators: - Aortic lipid deposition: Oil Red O staining showed intimal lipid aggregation, without obvious fibrous plaques; 3. Molecular indicators: - Upregulated expression of aortic inflammatory genes (VCAM-1, ICAM-1).

*Precautions：

*References：Xiangdong L,et,al. Animal models for the atherosclerosis research: a review. Protein Cell. 2011 Mar;2(3):189-201.

SynonymsCholesteryl alcohol, Cholesterin
Chemical Properties
Molecular Weight386.65
FormulaC27H46O
Cas No.57-88-5
SmilesC[C@@]12[C@]([C@]3([C@@]([C@]4(C)C(=CC3)C[C@@H](O)CC4)(CC1)[H])[H])(CC[C@@]2([C@@H](CCCC(C)C)C)[H])[H]
Relative Density.1.06 g/cm3
Storage & Solubility Information
Storagestore at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
H2O: < 1 mg/mL (insoluble)
DMSO: < 1mg/mL (insoluble)
Ethanol: 11.42 mg/mL (29.54 mM), Sonication is recommended.
Solution Preparation Table
Ethanol
1mg5mg10mg50mg
1 mM2.5863 mL12.9316 mL25.8632 mL129.3159 mL
5 mM0.5173 mL2.5863 mL5.1726 mL25.8632 mL
10 mM0.2586 mL1.2932 mL2.5863 mL12.9316 mL
20 mM0.1293 mL0.6466 mL1.2932 mL6.4658 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:
TargetMol | Animal experiments For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent 10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.
Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSOTargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSOTargetMol | reagent stock solution to 400 μL PEG300TargetMol | reagent and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH2OTargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.
All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.
1 Enter information below:
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Keywords

unesterifiedtransportersSREBP2signalingRORαphosphorylationMRP1IRS-1insulinInhibitorinhibitexocyticEstrogenReceptorEstrogen Receptor/ERREstrogen ReceptorERRαERREndogenousMetaboliteEndogenous MetaboliteDiabetesCholesterolAKT
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