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MAPK Raf ZM 336372

ZM 336372

Catalog No. T1851   CAS 208260-29-1
Synonyms: Zinc00581684

ZM 336372 is a potent and selective c-Raf inhibitor.

ZM 336372, CAS 208260-29-1
Pack Size Availability Price/USD Quantity
5 mg In stock 59.00
10 mg In stock 98.00
25 mg In stock 185.00
50 mg In stock 336.00
100 mg In stock 605.00
1 mL * 10 mM (in DMSO) In stock 59.00
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Purity 97.24%
Purity 97.51%
Biological Description
Chemical Properties
Storage & Solubility Information
Description ZM 336372 is a potent and selective c-Raf inhibitor.
Targets&IC50 C-Raf
Kinase Assay In vitro kinase assay: c-Raf kinase activity is assayed directly in Sl9 cell lysates. Human c-Raf is activated in Sf9 cells by cotransfection from baculovirus vectors containing DNA encoding v-Ras and Lck in the absence of ZM 336372. The cell lysates are then assayed for c-Raf activity in the presence of increasing concentrations of ZM 336372.
Cell Research
Cells are exposed to various concentrations of ZM 336372 for 48 and 72 hours. After incubation, the medium is removed and cells are trypsinized. Cells are incubated on ice, and 2.5 μg/mL propidium iodide is added 5 minutes before flow cytometry. Data is acquired using a FACSCalibur benchtop flow cytometer using CellQuest acquisition and analysis software. Cytotoxicity is done using Cell Titer Glo Assay. Cell proliferation is measured using MTT assay.(Only for Reference)
Cell lines: H727 and BON
Synonyms Zinc00581684
Purity 97.24%
Molecular Weight 389.45
Formula C23H23N3O3
CAS No. 208260-29-1

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 72 mg/mL (184.9 mM)

Ethanol: 2 mg/mL (5.13 mM)

Water: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Hall-Jackson CA, et al. Chem Biol, 1999, 6(8), 559-568. 2. Wartenberg M, et al. Int J Cancer, 2003, 104(3), 274-282. 3. Van Gompel JJ, et al. Mol Cancer Ther, 2005, 4(6), 910-917. 4. Kappes A, et al. J Surg Res, 2006, 133(1), 42-55. 5. Deming D, et al. J Gastrointest Surg, 2008, 12(5), 852-857.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Apoptosis Compound Library Autophagy Compound Library MAPK Inhibitor Library Tyrosine kinase inhibitor library Kinase Inhibitor Library Anti-cancer Metabolism Compound Library CNS-Penetrant Compound Library Anti-obesity Compound Library Target-Focused Phenotypic Screening Library HIF-1 Signaling Pathway Compound Library Anti-Pancreatic Cancer Compound Library Glutamine Metabolism Compound Library

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Answers to questions you may have can be found in the Inhibitor Handling Instructions. Topics include how to prepare stock solutions, how to store Products, and issues that need special attention for cell-based assays and animal experiments.