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Z-FA-FMK

Catalog No. T6738 CAS 197855-65-5

Z-FA-FMK can irreversibly inhibit cysteine protease and also inhibit effector caspases.

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Z-FA-FMK, CAS 197855-65-5

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Purity 98%

Biological Description

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Storage & Solubility Information

Description

Z-FA-FMK can irreversibly inhibit cysteine protease and also inhibit effector caspases.

Cell Research

T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester. (Only for Reference)

Molecular Weight	386.42
Formula	C21H23FN2O4
CAS No.	197855-65-5

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 71 mg/mL (183.7 mM)

H2O: <1 mg/mL

Ethanol: 32 mg/mL (82.8 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature

1. Rasnick D. Anal Biochem. 1985, 149(2), 461-465. 2. van Noorden CJ, et al. Biochem Biophys Res Commun. 1991, 178(1), 178-184. 3. Schotte P, et al. J Biol Chem. 2001, 276(24), 21153-21157. 4. Lawrence CP, et al. J Immunol. 2006, 177(6), 3827-3836. 5. Kim M, et al. Antivir Ther. 2010, 15(6), 897-905.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Fluorochemical Library Apoptosis Compound Library Target-Focused Phenotypic Screening Library Anti-Aging Compound Library Anti-Infection Compound Library Bioactive Compound Library

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Related compounds with same targets

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.