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WS3

Name: WS3
Brand: TargetMol
SKU: T2055
Price: 35 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2055 Copy Product Info

Purity: 99.94%

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WS3, a β cell proliferation inducer, regulates Erb3 binding protein-1 (EBP1) and the IκB kinase pathway.

Cas No. 1421227-52-2

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Pack Size	Price	USA Stock	Global Stock
5 mg	$35	In Stock	In Stock
10 mg	$64	In Stock	In Stock
25 mg	$123	In Stock	In Stock
50 mg	$198	In Stock	In Stock
100 mg	$328	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$55	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.94%

Appearance:Solid

Color:White to Yellow

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	WS3, a β cell proliferation inducer, regulates Erb3 binding protein-1 (EBP1) and the IκB kinase pathway.
Targets & IC50	Proliferation (β cells):28 nM(ec50)
In vivo	In the mouse β-cell line R7T1, WS3 induces cell proliferation with an EC50 of 0.074 μM. Additionally, WS3 reversibly induces cell proliferation in primary embryonic and adult retinal pigment epithelial cells.
Cell Research	R7T1 cells are growth-arrested by removal of doxycycline for 2 days and plated into 384-well plates at a density of 3,000 cells/well in growth medium. Β cell proliferation is assessed using CellTiter Glo after 4 days. Fold increase in cell number is calculated by normalizing compound-treated wells to the median of DMSO-treated wells. (Only for Reference)

Chemical Properties

Molecular Weight	569.58
Formula	C₂₈H₃₀F₃N₇O₃
Cas No.	1421227-52-2
Smiles	C(C1=C(C(F)(F)F)C=C(NC(NC2=CC=C(OC=3C=C(NC(=O)C4CC4)N=CN3)C=C2)=O)C=C1)N5CCN(C)CC5
Relative Density.	1.423 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 250 mg/mL (438.92 mM), Sonication is recommended.

Ethanol: 74 mg/mL (129.92 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Saline: < 10 mg/mL (17.56 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 10 mg/mL (17.56 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.7557 mL	8.7784 mL	17.5568 mL	87.7840 mL
5 mM	0.3511 mL	1.7557 mL	3.5114 mL	17.5568 mL
10 mM	0.1756 mL	0.8778 mL	1.7557 mL	8.7784 mL
20 mM	0.0878 mL	0.4389 mL	0.8778 mL	4.3892 mL
50 mM	0.0351 mL	0.1756 mL	0.3511 mL	1.7557 mL
100 mM	0.0176 mL	0.0878 mL	0.1756 mL	0.8778 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc