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WAY-100635 Monomaleate

Catalog No. T2631 CAS 1092679-51-0

WAY-100635 is a specific and effective 5-HT receptor antagonist (IC50=0.95 nM).

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WAY-100635 Monomaleate Chemical Structure

WAY-100635 Monomaleate, CAS 1092679-51-0

Pack Size	Availability	Price/USD
2 mg	In stock	$ 38.00
5 mg	In stock	$ 61.00
10 mg	In stock	$ 96.00
25 mg	In stock	$ 189.00
50 mg	In stock	$ 351.00
100 mg	In stock	$ 522.00
200 mg	In stock	$ 755.00
500 mg	In stock	$ 1,170.00
1 mL * 10 mM (in DMSO)	In stock	$ 72.00

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Purity: 100%

Purity: 99.68%

Purity: 99.46%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	WAY-100635 is a specific and effective 5-HT receptor antagonist (IC50=0.95 nM).
Targets&IC50	5-HT:0.95 nM
Kinase Assay	Enzyme activity assay: To determine the IC50 values of HM781-36B for kinase inhibition, enzymes of EGFR, HER2, and HER4 are expressed as recombinant proteins in Sf9 insect cells. Enzyme selectivity screening is then performed using a tyrosine kinase assay kit. Briefly, the reactions are performed in 96 well polystyrene round-bottomed plates containing kinase buffer composed of 100 mM HEPES (pH 7.4), 25 mM MgCl2, 10 mM MnCl2 and 250 μM Na3VO4. The reactions are initiated by the addition of 100 ng/assay enzyme, 100 μM ATP, and 10 ng/mL poly(Glu, Tyr). After 1 h of incubation at room temperature, the reactions are terminated by adding 6 mM EDTA solution and then anti-phosphotyrosine antibody, PTK Green Tracer, and FP dilution buffer mixtures. The fluorescence polarization values are then measured after 30 min at room temperature using a Victor3 microplate reader. Finally, the IC50 values were calculated using the following equation: Y = bottom + (top–bottom)/(1 + 10(X-logIC50)).
Cell Research	Extracellular recordings are made with glass microelectrodes filled with 2 M NaC1 (12 MΩ-15 MΩ). Cells are identified as 5-HT neurons according to the following criteria: biphasic action potentials of 2 msec to 3 msec in duration, slow (0.5 Hz - 2.0 Hz) and regular pattern of discharge. Firing is evoked in the otherwise silent neurons by adding the alpha-l adrenergic agonist phenylephrine (3 μM) to the superfusing ACSF. Baseline activity is recorded for at least 10 minutes before application of the different drugs. The electric signals are fed into a high-input impedance amplifier, an oscilloscope and an electronic ratemeter triggered by individual action potentials connected to an A/D converter and a personal computer. Using dedicated software, the integrated firing rate is recorded, computed and displayed on a chart recorder as consecutive 10-sec samples. The effects of agonists are evaluated by comparing the mean discharge frequency recorded during the 2 minutes that preceded WAY 100635 application with that recorded at the peak of WAY 100635 action (usually 2-5 minutes after the beginning of application). When the agonists are applied in the presence of the antagonist, the effect of the agonist is compared to baseline firing rate and to the frequency recorded during superfusion of the antagonist alone. The antagonist is left to equilibrate for 10 minutes to 25 minutes before retesting of the action of agonists. (Only for Reference)

Molecular Weight	538.64
Formula	C25H34N4O2·C4H4O4
CAS No.	1092679-51-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 79 mg/mL (146.7 mM)

DMSO: 79 mg/mL (146.7 mM)

References and Literature

1. Corradetti R, et al. J Pharmacol Exp Ther. 1996, 278(2), 679-688. 2. Fletcher A, et al. Behav Brain Res. 1996, 73(1-2), 337-353. 3. Hall H, et al. Brain Res. 1997, 745(1-2), 96-108. 4. Forster EA, et al. Eur J Pharmacol. 1995, 281(1), 81-88. 5. Laporte AM, et al. Eur J Pharmacol. 1994, 271(2-3), 505-514.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library GPCR Compound Library Anti-Neurodegenerative Disease Compound Library Anti-Obesity Compound Library Anti-Metabolism Disease Compound Library Bioactive Compounds Library Max Antidepressant Compound Library Bioactive Compound Library Neurotransmitter Receptor Compound Library Anti-Cancer Compound Library

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Keywords

WAY-100635 Monomaleate 1092679-51-0 GPCR/G Protein Neuroscience 5-HT Receptor Dopamine Receptor WAY 100635 WAY 100635 Maleate WAY-100635 Maleate WAY-100635 WAY100635 Maleate inhibit 5-hydroxytryptamine Receptor Inhibitor WAY100635 Serotonin Receptor inhibitor

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