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Cytoskeletal Signaling Akt Triciribine

Triciribine

Catalog No. T6065   CAS 35943-35-2
Synonyms: NSC 154020, TCN, API-2, VD-0002, Tricyclic nucleoside, VD-0002, Tricyclic nucleoside, TCN, NSC 154020 API-2

Triciribine is a DNA synthesis inhibitor, and it also inhibits Akt and HIV-1/2.

Triciribine, CAS 35943-35-2
Pack Size Availability Price/USD Quantity
2 mg In stock 50.00
5 mg In stock 93.00
10 mg In stock 128.00
25 mg In stock 259.00
50 mg In stock 445.00
100 mg In stock 801.00
1 mL * 10 mM (in DMSO) In stock 93.00
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Purity 99.87%
Purity 100.00%
Biological Description
Chemical Properties
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Description Triciribine is a DNA synthesis inhibitor, and it also inhibits Akt and HIV-1/2.
Targets&IC50 Akt ,   HIV-1
In vivo 1 mg/kg/day i.p. treated Triciribine inhibits OVCAR3, OVCAR8 and PANC1 tumor growth, which overexpressing Akt, by 90%, 88% and 80% in nude mice, respectively. However, Triciribine has little effect on the growth of OVCAR5 and COLO357 cells. [4]
Kinase Assay Akt Phosphorylation Changes Assay: Cells are grown to 80%–90% confluency and stimulated for 5–10 minutes with 1–10 ng/mL of epidermal growth factor or platelet derived growth factor (PDGF)–AA with or without 10–20 mM of U0126 or LY-294002. Protein lysates (5–20 μg) are separated by 12%–15% SDS PAGE and analyzed by Western blot for Akt, phosphorylated Akt (phospho-Ser 473), MAPK, and phosphorylated MAPK (p44/42 phospho-Thr202/Tyr204) antibodies (1:1000).
Cell Research
Triciribine is evaluated for cytotoxicity by seeding CEM-SS cells at a density of 1 × 104 cells/well in growth medium, using a 96-well flat-bottom plate. Serial fivefold dilutions of Triciribine are prepared in growth medium and added to the wells as a second overlay. After a 48-hours incubation at 37 °C, the cells are pulse labeled with [3H]dThd (1 μCi per well, specific activity 20 Ci/mmol) for 6 hours and the cells are harvested to measure total DNA synthesis.(Only for Reference)
Cell lines: CEM-SS cells
Synonyms NSC 154020 , TCN , API-2 , VD-0002 , Tricyclic nucleoside , VD-0002 , Tricyclic nucleoside , TCN , NSC 154020 API-2
Purity 99.87%
Molecular Weight 320.3
Formula C13H16N6O4
CAS No. 35943-35-2

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

1eq. HCl: 32 mg/mL (100 mM)

DMSO: 32 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

1% DMSO/30% polyethylene glycol/1% Tween 80: 30 mg/mL

Citations

References and Literature
1. Gursel DB, et al, Nero Oncol, 2011, 13(6), 610-621. 2. Kucera LS, et al, AIDS Res Hum Retroviruses, 1993, 9(4), 307-314. 3. Dieterle A, et al, Int J Cancer , 2009, 125(4), 932-941. 4. Yang L, et al, Cancer Res, 2004, 64(13), 4394-4399. 5. Abdalla M, et al. The Akt inhibitor, triciribine, ameliorates chronic hypoxia-induced vascular pruning and TGFβ-induced pulmonary fibrosis. Br J Pharmacol. 2015 Aug;172(16):4173-88.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Clinical Compound Library Stem cell Differentiation Compound Library Anti-infection Compound Library Autophagy Compound Library PI3K-AKT-mTOR Compound Library Anti-virus Compound Library Immunology/Inflammation Compound Library DNA Damage & Repair Compound Library Kinase Inhibitor Library Anti-cancer Clinical Compound Library Anti-cancer Metabolism Compound Library Promoting Cancer Cell Differentiation Compound Library Anti-obesity Compound Library Anti-aging Compound Library Hematonosis Compound Library Anti-cancer Active Compound library Anti-cancer Drug library Drug Repurposing Library HIF-1 Signaling Pathway Compound Library Nucleotide Compound Library Anti-Lung Cancer Compound Library Glycometabolism Compound Library Anti-Breast Cancer Compound Library Anti-Pancreatic Cancer Compound Library Cytoskeletal Signaling Pathway Compound Library Glutamine Metabolism Compound Library

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