Home Tools
Log in
Cart

Torin 2

Catalog No. T6100   CAS 1223001-51-1

Torin 2(IC50=0.25 nM), a specific and effective mTOR inhibitor, is the 800-fold greater specific activity for mTOR than PI3K and improves pharmacokinetic properties. The EC50 of Torin 2 for ATM/ATR/DNA-PK inhibition is 28 nM/35 nM/118 nM, respectively.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Torin 2 Chemical Structure
Torin 2, CAS 1223001-51-1
Pack Size Availability Price/USD Quantity
2 mg In stock $ 35.00
5 mg In stock $ 56.00
10 mg In stock $ 93.00
25 mg In stock $ 169.00
50 mg In stock $ 271.00
100 mg In stock $ 401.00
200 mg In stock $ 597.00
500 mg In stock $ 953.00
1 mL * 10 mM (in DMSO) In stock $ 62.00
Bulk Inquiry
Get quote
Select Batch  
Purity: 99.47%
Purity: 99%
Purity: 98.31%
Contact us for more batch information
Biological Description
Chemical Properties
Storage & Solubility Information
Description Torin 2(IC50=0.25 nM), a specific and effective mTOR inhibitor, is the 800-fold greater specific activity for mTOR than PI3K and improves pharmacokinetic properties. The EC50 of Torin 2 for ATM/ATR/DNA-PK inhibition is 28 nM/35 nM/118 nM, respectively.
Targets&IC50 mTOR:0.25 nM, ATR:35 nM(EC50), DNA-PK:118 nM(EC50), ATM:28 nM(EC50)
In vitro In Th-MYCN mice, Torin 2 (20 mg/kg) eradicated MYCN tumors, decreased MYCN protein levels, and induced apoptosis. In liver microsome stability assays, Torin 2 demonstrated greater than 95% pharmacological response with a half-life of 11.7 minutes. The best bioavailability of Torin 2 in male Swiss albino mice, whether administered intravenously or orally, was 51%, with a half-life of 0.72 hours and a low clearance rate of 19.6 mL/min/kg.
In vivo In MZ-CRC-1 and TT cells, Torin 2 (at concentrations < 50 nM) significantly reduces cell viability, while at 100 nM, it notably decreases cell migration. Torin 2 inhibits mTORC1, thus activating TFEB by promoting its nuclear translocation, with an EC50 value of 1.666 mM. Its mechanism involves a similar binding pattern to PI3Kγ, with V882 serving as a pivotal hinge binding site. This interaction is further stabilized by three hydrogen bonds from Y867, D841, and D964 targeting amino benzyl side chains, mirroring the interaction seen with mTOR’s Y2225, D2195, and D2357.
Kinase Assay mTOR and PI3K Cellular Assays: Cellular IC50 values for mTOR are determined using p53?/? MEFs. Cells are treated with vehicle or increasing concentrations of Torin 2 for 1 h and then lyse. Phosphorylation of S6K1 Thr-389 is monitored by immunoblotting using a phospho-specific antibody. Meanwhile, cellular IC50 values for PI3Ka are determined based on phosphorylation of Akt Thr-308 in p53?/?/mLST8?/? MEFs or human PC3 cells expressing the S473D mutant of Akt1.
Cell Research For viability, MZ-CRC-1 and TT cells are seeded in quadruplicate in 96-well plates (1.0×104 cells per well) in culture media with 2.5% and 4% FBS, respectively. After 24 hours, cells are treated with Torin 2. At the indicated time point, cells are incubated for 3 hours with 10 μL of CellTiter96 AQueous One solution in 100 μL of culture media and absorbance is measured at 490 nm.(Only for Reference)
Molecular Weight 432.4
Formula C24H15F3N4O
CAS No. 1223001-51-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 8.7 mg/mL (20 mM)

TargetMolReferences and Literature

1. Liu Q, et al. J Med Chem, 2011, 54(5), 1473-1480. 2. Settembre C, et al. EMBO J, 2012, 31(5), 1095-1108. 3. Tamburrino A, et al. Clin Cancer Res, 2012, 18(13), 3532-3540. 4. Berry T, et al. Cancer Cell, 2012, 22(1), 117-130. 5. Liu Q, et al. Cancer Res, 2013, 73(8), 2574-2586. 6. Wang X, et al. mTORC signaling in hematopoiesis. Int J Hematol. 2016 May;103(5):510-8.

TargetMolCitations

1. Jiang X, Liu K, Jiang H, et al.SLC7A14 imports GABA to lysosomes and impairs hepatic insulin sensitivity via inhibiting mTORC2.Cell Reports.2023, 42(1): 111984.

Related compound libraries

This product is contained In the following compound libraries:
Highly Selective Inhibitor Library Tyrosine Kinase Inhibitor Library Neuronal Differentiation Compound Library Anti-Diabetic Compound Library Apoptosis Compound Library Bioactive Compound Library Anti-Cancer Metabolism Compound Library Metabolism Compound Library Anti-Obesity Compound Library Fluorochemical Library

Related Products

Related compounds with same targets
KH16 Agrimonolide Anticancer agent 130 Y-27632 Taurodeoxycholic acid sodium hydrate Antitumor agent-97 SCFSkp2-IN-2 Corilagin

TargetMolDose Conversion

You can also refer to dose conversion for different animals. More

TargetMol In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Dosage
mg/kg
Average weight of animals
g
Dosing volume per animal
ul
Number of animals
Step Two: Enter the in vivo formulation
% DMSO
%
% Tween 80
% ddH2O
Calculate Reset

TargetMolCalculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
=
X
X

Molarity Calculator allows you to calculate the

  • Mass of a compound required to prepare a solution of known volume and concentration
  • Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

X
=
X

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

=
/

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Torin 2 1223001-51-1 Apoptosis Autophagy DNA Damage/DNA Repair PI3K/Akt/mTOR signaling DNA-PK ATM/ATR mTOR DNA-dependent protein kinase Torin-2 inhibit Mammalian target of Rapamycin Torin2 Inhibitor inhibitor

 

TargetMol