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Membrane transporter/Ion channel Potassium Channel Tolbutamide

Tolbutamide

Catalog No. T1054   CAS 64-77-7
Synonyms: HLS 831

Tolbutamide is a sulphonylurea hypoglycemic agent with actions and uses similar to those of CHLORPROPAMIDE.

Tolbutamide, CAS 64-77-7
Pack Size Availability Price/USD Quantity
5 mg In stock 16.00
10 mg In stock 20.00
25 mg In stock 26.00
50 mg In stock 33.00
100 mg In stock 40.00
200 mg In stock 50.00
500 mg In stock 60.00
1 g In stock 75.00
1 mL * 10 mM (in DMSO) In stock 54.00
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Tolbutamide is a sulphonylurea hypoglycemic agent with actions and uses similar to those of CHLORPROPAMIDE.
Targets&IC50 Potassium Channel,  
Kinase Assay cAMP kinase assay: Diced epididymal fat pads from fed Wistar rats (175-225 gm) are obtained after decapitation and incubated at 37 °C for two hours in Krebs-bicarbonate buffer containing 1.27 mM CaCl2. When added, Tolbutamide is present only during the incubation. After incubation fat pads are rinsed and sonicated in cold Krebs-bicarbonate buffer. The aqueous supematants from centrifugation at 50,000 × g for 30 minutes at 4 °C contained 0.75 to 1.25 mg protein per mL and are assayed for cyclic AMP-stimulated protein kinase activity. The assay is performed in 0.2 mL with these additions, 10 μmoles sodium glycerofiosphate pH 7.0, 2 μmoles sodium fluoride, 0.4 μmoles theophylline, 0.1 μmoles ethylene glyool bis (β-aminoethyl ether)-N, N'-tetraaoetic acid, 3 μmoles magnesium chloride, 0.3 mg mixed histone, 2 nmoles (γ- 32P) ATP, 1 nmoles cyclic AMP when indicated, and 0.05 ml of supernatant.
Cell Research
C6 glioma cells are incubated in serum-free DMEM at 37 °C for at least 24 hours before each experiment. Tolbutamide (400 μM) is incubated for 24 hours in serum-free medium. Incubations are performed at 37 °C in an atmosphere of 95% air/5% CO2 with 90–95% humidity. (Only for Reference)
Cell lines: C6 glioma cells
Animal Research
Animal Model: albino Wistar rats (200-300 g)
Synonyms HLS 831
Purity 99.65%
Molecular Weight 270.35
Formula C12H18N2O3S
CAS No. 64-77-7

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 50 mg/mL (184.9 mM)

Ethanol: 50 mg/mL (184.9 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Wray HL, et al, Biochem Biophys Res Commun. 1973, 53(1), 291-294. 2. Sanchez-Alvarez R, et al, Glia, 2006, 54(2), 125-134. 3. Schwanstecher C, et al, Mol Pharmacol, 1992, 41(3), 480-486. 4. Avame H, et al, Am J Physiol, 1995, 268(3 Pt 1), 392-396. 5. Joost HG, et al, Biochem Pharmacol, 1982, 31(7), 1227-1231.

Related compound libraries

This product is contained In the following compound libraries:
Approved Drug Library Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Anti-diabetic Compound Library Autophagy Compound Library Ion Channel Inhibitor Library FDA-approved Drug Library Anti-Metabolism Disease Compound Library Killers Collection

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