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Tolbutamide

Catalog No. T1054   CAS 64-77-7
Synonyms: HLS 831, 甲苯磺丁脲

Tolbutamide is a sulphonylurea hypoglycemic agent with actions and uses similar to those of CHLORPROPAMIDE.

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Tolbutamide, CAS 64-77-7
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Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Tolbutamide is a sulphonylurea hypoglycemic agent with actions and uses similar to those of CHLORPROPAMIDE.
Targets&IC50 cyclic AMP-stimulated protein kinase:4 mM
Kinase Assay cAMP kinase assay: Diced epididymal fat pads from fed Wistar rats (175-225 gm) are obtained after decapitation and incubated at 37 °C for two hours in Krebs-bicarbonate buffer containing 1.27 mM CaCl2. When added, Tolbutamide is present only during the incubation. After incubation fat pads are rinsed and sonicated in cold Krebs-bicarbonate buffer. The aqueous supematants from centrifugation at 50,000 × g for 30 minutes at 4 °C contained 0.75 to 1.25 mg protein per mL and are assayed for cyclic AMP-stimulated protein kinase activity. The assay is performed in 0.2 mL with these additions, 10 μmoles sodium glycerofiosphate pH 7.0, 2 μmoles sodium fluoride, 0.4 μmoles theophylline, 0.1 μmoles ethylene glyool bis (β-aminoethyl ether)-N, N'-tetraaoetic acid, 3 μmoles magnesium chloride, 0.3 mg mixed histone, 2 nmoles (γ- 32P) ATP, 1 nmoles cyclic AMP when indicated, and 0.05 ml of supernatant.
Cell Research C6 glioma cells are incubated in serum-free DMEM at 37 °C for at least 24 hours before each experiment. Tolbutamide (400 μM) is incubated for 24 hours in serum-free medium. Incubations are performed at 37 °C in an atmosphere of 95% air/5% CO2 with 90–95% humidity. (Only for Reference)
Synonyms HLS 831, 甲苯磺丁脲
Molecular Weight 270.35
Formula C12H18N2O3S
CAS No. 64-77-7

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 50 mg/mL (184.9 mM)

Ethanol: 50 mg/mL (184.9 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Wray HL, et al, Biochem Biophys Res Commun. 1973, 53(1), 291-294. 2. Sanchez-Alvarez R, et al, Glia, 2006, 54(2), 125-134. 3. Schwanstecher C, et al, Mol Pharmacol, 1992, 41(3), 480-486. 4. Avame H, et al, Am J Physiol, 1995, 268(3 Pt 1), 392-396. 5. Joost HG, et al, Biochem Pharmacol, 1982, 31(7), 1227-1231.

Citations

1. Guo J, Xu Y, Chen L J, et al. Gut Microbiota and Host Cyp450s Co-contribute to Pharmacokinetic Variability in Mice With Non-Alcoholic Steatohepatitis: Vary From Drug to Drug. Journal of Advanced Research. 2021

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Compound Library Autophagy Compound Library Anti-Obesity Compound Library Inhibitor Library Ion Channel Inhibitor Library Anti-Metabolism Disease Compound Library Drug Repurposing Compound Library Anti-Cancer Approved Drug Library FDA-Approved Drug Library Toxic Compound Library

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Keywords

Tolbutamide 64-77-7 离子通道 自噬 Autophagy Potassium Channel inhibit Inhibitor KcsA HLS 831 inhibitor