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Tolbutamide

Catalog No. T1054 CAS 64-77-7

Synonyms: HLS 831

Tolbutamide is a sulphonylurea hypoglycemic agent with actions and uses similar to those of CHLORPROPAMIDE.

Tolbutamide, CAS 64-77-7

Pack Size	Availability	Price/USD
5 mg	In stock	16.00
10 mg	In stock	20.00
25 mg	In stock	26.00
50 mg	In stock	33.00
100 mg	In stock	40.00
200 mg	In stock	50.00
500 mg	In stock	60.00
1 g	In stock	75.00
1 mL * 10 mM (in DMSO)	In stock	54.00

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Purity 99.65%

HNMR HPLC COA Datasheet MSDS

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Tolbutamide is a sulphonylurea hypoglycemic agent with actions and uses similar to those of CHLORPROPAMIDE.
Targets&IC50	Potassium Channel,
Kinase Assay	cAMP kinase assay: Diced epididymal fat pads from fed Wistar rats (175-225 gm) are obtained after decapitation and incubated at 37 °C for two hours in Krebs-bicarbonate buffer containing 1.27 mM CaCl2. When added, Tolbutamide is present only during the incubation. After incubation fat pads are rinsed and sonicated in cold Krebs-bicarbonate buffer. The aqueous supematants from centrifugation at 50,000 × g for 30 minutes at 4 °C contained 0.75 to 1.25 mg protein per mL and are assayed for cyclic AMP-stimulated protein kinase activity. The assay is performed in 0.2 mL with these additions, 10 μmoles sodium glycerofiosphate pH 7.0, 2 μmoles sodium fluoride, 0.4 μmoles theophylline, 0.1 μmoles ethylene glyool bis (β-aminoethyl ether)-N, N'-tetraaoetic acid, 3 μmoles magnesium chloride, 0.3 mg mixed histone, 2 nmoles (γ- 32P) ATP, 1 nmoles cyclic AMP when indicated, and 0.05 ml of supernatant.
Cell Research	C6 glioma cells are incubated in serum-free DMEM at 37 °C for at least 24 hours before each experiment. Tolbutamide (400 μM) is incubated for 24 hours in serum-free medium. Incubations are performed at 37 °C in an atmosphere of 95% air/5% CO2 with 90–95% humidity. (Only for Reference) Cell lines: C6 glioma cells
Animal Research	Animal Model: albino Wistar rats (200-300 g)

Synonyms	HLS 831
Purity	99.65%
Molecular Weight	270.35
Formula	C12H18N2O3S
CAS No.	64-77-7

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 50 mg/mL (184.9 mM)

Ethanol: 50 mg/mL (184.9 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature

1. Wray HL, et al, Biochem Biophys Res Commun. 1973, 53(1), 291-294. 2. Sanchez-Alvarez R, et al, Glia, 2006, 54(2), 125-134. 3. Schwanstecher C, et al, Mol Pharmacol, 1992, 41(3), 480-486. 4. Avame H, et al, Am J Physiol, 1995, 268(3 Pt 1), 392-396. 5. Joost HG, et al, Biochem Pharmacol, 1982, 31(7), 1227-1231.

Related compound libraries

This product is contained In the following compound libraries：

Approved Drug Library Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Anti-diabetic Compound Library Autophagy Compound Library Ion Channel Inhibitor Library FDA-approved Drug Library Anti-Metabolism Disease Compound Library Killers Collection

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Answers to questions you may have can be found in the Inhibitor Handling Instructions. Topics include how to prepare stock solutions, how to store Products, and issues that need special attention for cell-based assays and animal experiments.

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.