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Sempervirine

Catalog No. TN2202 CAS 6882-99-1

Sempervirine can unwind circular DNA, it shows selective inhibition of in vitro synthesis of cancer DNA .

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Sempervirine, CAS 6882-99-1

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Sempervirine can unwind circular DNA, it shows selective inhibition of in vitro synthesis of cancer DNA .

In vitro

The high template in vitro activity of native DNA from cancerous mammalian and plant tissues, compared to DNA from healthy tissues, enabled us to select substances which selectively inhibit cancer DNA synthesis. Among them, alstonine, serpentine, Sempervirine and flavopereirine, all alkaloids which belong to the Beta-carboline class, distinguish cancer DNA from healthy tissue DNA inhibit DNA in vitro synthesis when native DNA from different cancerous tissues or cells is used as template. They have practically no effect on DNA from healthy tissues. The inhibitory effect of alkaloids is due to their capacity to form an 'alkaloid-cancer DNA' complex which has been characterized by use of the Sephadex column.

Molecular Weight	272.4
Formula	C19H16N2
CAS No.	6882-99-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: <2.73 mg/mL (10 mM), Need ultrasound

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Selective inhibition of in vitro synthesis of cancer DNA by alkaloids of beta-carboline class.Exp Cell Biol. 1982;50(2):79-87.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
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The answer of 19.713 mg appears in the Mass box

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Sempervirine 6882-99-1 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis Inhibitor inhibitor inhibit

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