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Probenecid

Catalog No. T0457   CAS 57-66-9
Synonyms: Benemid

Probenecid (Benemid) is a benzoic acid derivative with antihyperuricemic property.

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Probenecid Chemical Structure
Probenecid, CAS 57-66-9
Pack Size Availability Price/USD Quantity
1 g In stock $ 50.00
1 mL * 10 mM (in DMSO) In stock $ 50.00
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Purity: 99.95%
Purity: 98.88%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Probenecid (Benemid) is a benzoic acid derivative with antihyperuricemic property.
Cell Research Probenecid is dissolved at 500 mM in 1 N NaOH and titrated to pH 7.0[1]. HEK-293T cells are transfected with hTAS2R expression constructs using Lipofectamine 2000 in poly-lysine coated, black 384-well plates with clear bottoms and incubated for 22 hours at 37°C. Growth media is removed and cells are washed twice with HBSS containing 20 mM HEPES, then loaded with a calcium indicator dye in HBSS containing 20 mM HEPES (Calcium 4 Assay kit) with or without 1 mM Probenecid. Cells are incubated at 37°C for 1 hour in the presence of both dye and Probenecid, then moved to a Flexstation II-384 set for 32°C. After a 15-minute temperature equilibration (without washout), indicated compounds are injected (at t=~25 seconds) and fluorescence is measured for 100 to 180 seconds, reading every 3 seconds. Data sets are analyzed and represented as % over baseline signal using Prism 5.0 software. For Schild plots, replicates of raw calcium flux values are expressed as % over baseline signal. The mean value at 36 seconds (corresponding to the maximum flux signal) for each concentration of TAS2R ligand in the presence of the indicated concentration of Probenecid is plotted against the log of ligand concentration. Data points are fit using non-linear regression in GraphPad Prism[1].
Synonyms Benemid
Molecular Weight 285.36
Formula C13H19NO4S
CAS No. 57-66-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 53 mg/mL (185.7 mM)

Ethanol: 21 mg/mL (73.6 mM)

TargetMolReferences and Literature

1. McDonough PM, et al. Cell Calcium, 1989, 10(3), 171-180. 2. Bang S, et al. Neurosci Lett, 2007, 425(2), 120-125. 3. Bakos E, et al. Mol Pharmacol, 2000, 57(4), 760-768. 4. Greene TA, et al. PLoS One, 2011, 6(5), e20123. 5. Robbins N, et al. Cardiovasc Toxicol, 2012, 12(1), 1-9.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Drug Library Anti-Cancer Clinical Compound Library Anti-Cancer Approved Drug Library Autophagy Compound Library Drug-Fragment Library FDA-Approved & Pharmacopeia Drug Library Anti-Infection Compound Library Inhibitor Library Anti-Bacterial Compound Library Orally Active Compound Library

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Keywords

Probenecid 57-66-9 Membrane transporter/Ion channel Microbiology/Virology Proteases/Proteasome HIV Protease Antibacterial TRP/TRPV Channel Benemid Bacterial inhibit Inhibitor Human immunodeficiency virus TRP Channel HIV Transient receptor potential channels inhibitor

 

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