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PMSF

Catalog No. T0789   CAS 329-98-6
Synonyms: Phenylmethylsulfonyl fluoride, Benzylsulfonyl fluoride

Phenylmethanesulfonyl fluoride (PMSF (Phenylmethylsulfonyl fluoride)) is an enzyme inhibitor that inactivates IRC-50 arvin, subtilisin, and the fatty acid synthetase complex.

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PMSF Chemical Structure
PMSF, CAS 329-98-6
Pack Size Availability Price/USD Quantity
50 mg In stock $ 33.00
100 mg In stock $ 46.00
200 mg In stock $ 48.00
500 mg In stock $ 55.00
1 mL * 10 mM (in DMSO) In stock $ 50.00
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Purity: 99.9%
Purity: 98.93%
Purity: 97.75%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Phenylmethanesulfonyl fluoride (PMSF (Phenylmethylsulfonyl fluoride)) is an enzyme inhibitor that inactivates IRC-50 arvin, subtilisin, and the fatty acid synthetase complex.
In vitro Treatment with PMSF (intraperitoneal injection) in mice elicits cannabinoid-like effects, providing analgesia (ED50: 86 mg/kg), hypothermia (ED50: 224 mg/kg), and catalepsy (ED50: 206 mg/kg). When administered to Sprague-Dawley rats, PMSF induces a dose-dependent analgesic effect and significantly potentiates the analgesic effect of β-endorphin in vivo. By inhibiting fatty acid amide hydrolase (FAAH) activity, PMSF suppresses typical cannabinoid or Δ(9)-tetrahydrocannabinol-like effects in ICR mice. Pretreatment with 30 mg/kg PMSF before the injection of [3H]-labeled cannabinoids results in a notable increase in brain cannabinoid levels after 5 minutes compared to [3H]-THC. PMSF pretreatment at 30 mg/kg enhances the cannabinoid-induced effects on the tail-flick response (analgesic effect), locomotion, and spontaneous activity by 5, 8, and 10-fold respectively. Administering PMSF 12 hours before paraoxon (PSP) protects hens from delayed neurotoxicity, whereas administration 4 hours later exacerbates the neurotoxic effects. PMSF pretreatment also prevents organophosphate-induced delayed neuropathy in hens and inhibits neuropathilament degeneration induced by tri-ortho-tolyl phosphate.
In vivo PMSF is a specific inhibitor of phospholipase C involved in the hydrolysis of phosphatidylinositol. In the longitudinal smooth muscle of guinea pig ileum, PMSF (2 mM) almost completely inhibits carbachol-activated synthesis of phosphatidylinositol muscarinic, without affecting synthesis activated by potassium. PMSF transiently inhibits muscle contractions induced by both potassium and carbachol. As an acetylcholinesterase inhibitor, PMSF outperforms BSF (at 8 times the concentration of PMSF), with BSF being sixfold less effective. PMSF rapidly inhibits the activity of trypsin purified from the human pancreas and acetylcholinesterase in human erythrocytes but has a negligible effect on human trypsin. In Trypanosoma brucei, PMSF inhibits the addition of ethanolamine phosphate to the intermediate of glycosylphosphatidylinositol and the acylation of the glycosyl residue in the blood GPI intermediates as well as the addition of ethanolamine phosphate and the acylation of glycosyl in the procyclic form, with no inhibition observed on the latter in mammalian HeLa cells.
Synonyms Phenylmethylsulfonyl fluoride, Benzylsulfonyl fluoride
Molecular Weight 174.19
Formula C7H7FO2S
CAS No. 329-98-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 17.4 mg/mL (100 mM)

DMSO: 17.4 mg/mL (100 mM)

TargetMolReferences and Literature

1. Turini P, et al. J Pharmacol Exp Ther, 1969, 167(1), 98-104. 2. Pinsky C, et al. Life Sci, 1982, 31(12-13), 1193-1196. 3. Sekar MC, et al. Cell Calcium, 1984, 5(3), 191-203. 4. Güther ML, et al. J Biol Chem. 1994 Jul 15;269(28):18694-701. 5. Compton DR, et al. J Pharmacol Exp Ther, 1997, 283(3), 1138-1143. 6. Wen Q L, Yi H Q, Yang K, et al. Role of oncogene PIM-1 in the development and progression of papillary thyroid carcinoma: involvement of oxidative stress[J]. Molecular and Cellular Endocrinology. 2020: 111144. 7. Xu Guang, Ting Li, Jiayi Chen, Changyan Li, Haixin Zhao, Chengcheng Yao, Hua Dong et al. Autosomal dominant retinitis pigmentosa-associated gene PRPF8 is essential for hypoxia-induced mitophagy through regulating ULK1mRNA splicing [J]. Autophagy. 2018;14(10):1818-1830.

TargetMolCitations

1. Xu G, Li T, Chen J, et al. Autosomal dominant retinitis pigmentosa-associated gene PRPF8 is essential for hypoxia-induced mitophagy through regulating ULK1mRNA splicing. Autophagy. 2018, 14(10): 1818-1830 2. Li X, Zhang H, Qiao S, et al Melatonin administration alleviates 2,2,4,4-tetra-brominated diphenyl ether (PBDE-47)-induced necroptosis and secretion of inflammatory factors via miR-140-5p/TLR4/NF-κB axis in fish kidney cells. Fish & Shellfish Immunology. 2022 3. Wen Q L, Yi H Q, Yang K, et al. Role of oncogene PIM-1 in the development and progression of papillary thyroid carcinoma: involvement of oxidative stress. Molecular and Cellular Endocrinology. 2021 Mar 1;523:111144. doi: 10.1016/j.mce.2020.111144 4. Song N, Guan X, Zhang S, et al.Discovery of a pyrrole-pyridinimidazole derivative as novel SIRT6 inhibitor for sensitizing pancreatic cancer to gemcitabine.Cell Death & Disease.2023, 14(8): 499. 5. Yan Y, Yu L, Chen B, et al.Mastoparan M Suppressed NLRP3 Inflammasome Activation by Inhibiting MAPK/NF-κB and Oxidative Stress in Gouty Arthritis.Journal of Inflammation Research.2023: 6179-6193.

Related compound libraries

This product is contained In the following compound libraries:
Highly Selective Inhibitor Library Bioactive Compound Library Target-Focused Phenotypic Screening Library NO PAINS Compound Library Anti-Metabolism Disease Compound Library Bioactive Compounds Library Max Covalent Inhibitor Library Fluorochemical Library Inhibitor Library Protease Inhibitor Library

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Keywords

PMSF 329-98-6 Proteases/Proteasome Serine Protease Cysteine Protease Phenylmethylsulfonyl fluoride inhibit Cathepsin Benzylsulfonyl Fluoride Benzylsulfonyl fluoride Inhibitor Phenylmethylsulfonyl Fluoride inhibitor

 

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