Antibody Type: Rabbit Polyclonal

Application: FCM

Reactivity: Human (predicted:Mouse,Rat,Dog)

IgG

Human (predicted:Mouse,Rat,Dog)

1. Blank control: A549 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μL in 100 μL 1X PBS containing 0.5% BSA (green).
2. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-Neutrophil Elastase antibody (TMAB-00603)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

FCM

FCM: 1μg/Test

Polyclonal

KLH conjugated synthetic peptide: human Neutrophil Elastase/ELANE

Human

1991

Calcium Signaling,Elastin,Other Enzymes,Defensin,Angiogenic factors ELISA kits,Adhesion molecules ELISA kits,NK Cells,Macrophage / Inflamm.,Regulatory,Extracellular matrix