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Anti-ELANE Polyclonal Antibody
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Catalog No. TMAB-00602
Alias PMN elastase, Neutrophil elastase, Medullasin, Human leukocyte elastase (HLE), Elastase-2, ELANE, ELA2, Bone marrow serine protease
Anti-ELANE Polyclonal Antibody is a Rabbit antibody targeting ELANE. Anti-ELANE Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-ELANE Polyclonal Antibody
Anti-ELANE Polyclonal Antibody
😃Good
Catalog No. TMAB-00602Alias PMN elastase, Neutrophil elastase, Medullasin, Human leukocyte elastase (HLE), Elastase-2, ELANE, ELA2, Bone marrow serine protease
Anti-ELANE Polyclonal Antibody is a Rabbit antibody targeting ELANE. Anti-ELANE Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-ELANE Polyclonal Antibody is a Rabbit antibody targeting ELANE. Anti-ELANE Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Alias | PMN elastase, Neutrophil elastase, Medullasin, Human leukocyte elastase (HLE), Elastase-2, ELANE, ELA2, Bone marrow serine protease |
| Ig Type | IgG |
| Reactivity | Mouse (predicted:Rat) |
| Verified Activity | Paraformaldehyde-fixed, paraffin embedded (mouse spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ELNE) Polyclonal Antibody, Unconjugated (TMAB-00602) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009]. |
| Immunogen | KLH conjugated synthetic peptide: human Neutrophil Elastase |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Calcium Signaling,Elastin,Other Enzymes,Defensin,Angiogenic factors ELISA kits,Adhesion molecules ELISA kits,NK Cells,Macrophage / Inflamm.,Regulatory,Extracellular matrix |
| Molecular Weight | Theoretical: 26 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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