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Anti-ELANE Polyclonal Antibody

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Catalog No. TMAB-00602

Anti-ELANE Polyclonal Antibody is a Rabbit antibody targeting ELANE. Anti-ELANE Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-ELANE Polyclonal Antibody

Anti-ELANE Polyclonal Antibody

😃Good
Catalog No. TMAB-00602
Anti-ELANE Polyclonal Antibody is a Rabbit antibody targeting ELANE. Anti-ELANE Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$222 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-ELANE Polyclonal Antibody is a Rabbit antibody targeting ELANE. Anti-ELANE Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Ig Type
IgG
Reactivity
Mouse (predicted:Rat)
Verified Activity
Paraformaldehyde-fixed, paraffin embedded (mouse spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ELNE) Polyclonal Antibody, Unconjugated (TMAB-00602) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundElastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins. The product of this gene hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. The enzyme may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, May 2009].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Neutrophil Elastase
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Calcium Signaling,Elastin,Other Enzymes,Defensin,Angiogenic factors ELISA kits,Adhesion molecules ELISA kits,NK Cells,Macrophage / Inflamm.,Regulatory,Extracellular matrix
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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