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Meisoindigo

Catalog No. T1882 CAS 97207-47-1

Synonyms: N-Methylisoindigotin, Methylisoindigotin, Natura-α, Dian III

Meisoindigo (Natura-α) is a derivative of indigo natural, might induces apoptosis and myeloid differentiation of acute myeloid leukemia (AML).

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Meisoindigo, CAS 97207-47-1

Pack Size	Availability	Price/USD
5 mg	In stock	$ 64.00
10 mg	In stock	$ 89.00
25 mg	In stock	$ 178.00
50 mg	In stock	$ 272.00
100 mg	In stock	$ 428.00
1 mL * 10 mM (in DMSO)	In stock	$ 70.00

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Purity: 98.19%

Purity: 98.03%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Meisoindigo (Natura-α) is a derivative of indigo natural, might induces apoptosis and myeloid differentiation of acute myeloid leukemia (AML).

Kinase Assay

GSK3 Scintillation Proximity Assay: The competition experiments are carried out in duplicate with 10 concentrations of the inhibitor in clear-bottomed microtiter plates. The biotinylated peptide substrate, biotin-AAEELDSRAGS(PO3H2)PQL, is added at a final concentration of 2 μM in an assay buffer containing 6 milliunits of recombinant human GSK3 (equal mix of both α and β), 12 mM MOPS, pH 7.0, 0.3 mM EDTA, 0.01% β-mercaptoethanol, 0.004% Brij 35, 0.5% glycerol, and 0.5 μg of bovine serum albumin/25 μl and preincubated for 10-15 min. The reaction is initiated by the addition of 0.04 μCi of [γ-33P]ATP and unlabeled ATP in 50 mM Mg(Ac)2 to a final concentration of 1 μM ATP and assay volume of 25 μl. Blank controls without peptide substrate are used. After incubation for 20 min at room temperature, each reaction is terminated by the addition of 25 μl of stop solution containing 5 mM EDTA, 50 μM ATP, 0.1% Triton X-100, and 0.25 mg of streptavidin-coated SPA beads corresponding to ～35 pmol of binding capacity. After 6 h the radioactivity is determined in a liquid scintillation counter. Inhibition curves are analyzed by non-linear regression using GraphPad Prism.

Synonyms	N-Methylisoindigotin, Methylisoindigotin, Natura-α, Dian III
Molecular Weight	276.29
Formula	C17H12N2O2
CAS No.	97207-47-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 18.33 mg/mL (66.36 mM)

References and Literature

1. Lee CC, et al. Leuk Lymphoma. 2010 May;51(5):897-905.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Drug Library Anti-Cancer Approved Drug Library Anti-Cancer Active Compound Library Anti-Cancer Clinical Compound Library Drug Repurposing Compound Library Natural Product Library Natural Product Library for HTS Anti-Cancer Compound Library Drug-induced Liver Injury (DILI) Compound Library Autophagy Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Meisoindigo 97207-47-1 Apoptosis NB4 G0/G1 Natura-a AML N-Methylisoindigotin Methylisoindigotin antitumor Natura-α Indirubin U937 myeloid Natura-alpha phase inhibit HL-60 acute leukemia Inhibitor Dian III primary inhibitor

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