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ML141

Catalog No. T2463 CAS 71203-35-5

Synonyms: CID-2950007

ML141 (CID-2950007) is an effective, specific and reversible non-competitive inhibitor of Rho family GTPase cdc42 (IC50: 200 nM).

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ML141, CAS 71203-35-5

Pack Size	Availability	Price/USD
1 mg	In stock	$ 33.00
2 mg	In stock	$ 46.00
5 mg	In stock	$ 66.00
10 mg	In stock	$ 112.00
25 mg	In stock	$ 236.00
50 mg	In stock	$ 428.00
100 mg	In stock	$ 612.00
500 mg	In stock	$ 1,320.00
1 mL * 10 mM (in DMSO)	In stock	$ 68.00

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Purity: 99.77%

Purity: 99.56%

Purity: 99.36%

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Description	ML141 (CID-2950007) is an effective, specific and reversible non-competitive inhibitor of Rho family GTPase cdc42 (IC50: 200 nM).
Targets&IC50	CDC42:200 nM
In vitro	In NOD/SCID mice carrying MDA-MB 231-derived tumors, ML141 (1 mg/day, intraperitoneally) inhibits the growth of these tumors by suppressing Cdc42, thereby enhancing the effectiveness of TMX. Additionally, ML141 (10 mg/kg, intraperitoneally) increases the mobilization of hematopoietic stem and progenitor cells induced by granulocyte colony-stimulating factors.
In vivo	ML141 significantly protects against apoptosis damage induced by metformin in neuroblastoma. It enhances the ability of caffeine to inhibit cell growth through the induction/suppression of cell death/division. Additionally, ML141 dose-dependently reduces the invasion of Klebsiella pneumoniae.
Kinase Assay	Equilibrium binding assay : Wild-type GST-Cdc42 (4 μM) is bound to GSH-beads overnight at 4°C. Cdc42 on GSH-beads is depleted of nucleotide by incubating with 10 mM EDTA containing buffer for 20 min at 30°C, washing twice with NP- HPS buffer, then re-suspended in the same buffer containing 1 mM EDTA/or 1 mM MgCl2, 1 mM DTT and 0.1% BSA. Cdc42 unbound sites are blocked by incubation of protein–bead complex for 15 min at RT. Thirty μL of this suspension is incubated with 20 mM inhibitor for 3 min at RT and added 30 μL of various concentrations of ice cold BODIPY-FL-GTP. Samples incubated at 4° C for 45 min and binding of fluorescent nucleotide to enzyme is measured using an Accuri flow cytometer. Raw data are exported and plotted using GraphPad Prism software.
Cell Research	Cells are incubated with 500 nM Calcein-AM and 1 µM PI for 15 min, after which live cells and dead cells (represented by positivity of Calcein-AM and PI staining, respectively) are counted utilizing the adherent cell Celigo™ cytometer. (Only for Reference)

Synonyms	CID-2950007
Molecular Weight	407.49
Formula	C22H21N3O3S
CAS No.	71203-35-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 40.8 mg/mL (100 mM)

References and Literature

1. Surviladze Z, et al. National Center for Biotechnology Information (US); 2010. 2. Chen HY, et al. EMBO Mol Med. 2013, 5(5), 723-736. 3. Kumar A, et al. Oncotarget. 2014, 5(22), 11709-11722. 4. Hsu CR, et al. Infect Immun. 2014. pii: IAI.02345-14. 5. Chen C, et al. DOI10.1007/s12185-014-1690-z. 6. . Marcut C F. Role of action dynamics in the cooperative maintenance of synaptic plasticity[D]. 2020 7. Lv J, Zeng J, Guo F, et al. Endothelial Cdc42 deficiency impairs endothelial regeneration and vascular repair after inflammatory vascular injury[J]. Respiratory research. 2018, 19(1): 27.

Citations

1. Lv J, Zeng J, Guo F, et al. Endothelial Cdc42 deficiency impairs endothelial regeneration and vascular repair after inflammatory vascular injury. Respiratory Research. 2018, 19(1): 1-11 2. Wang C, Hu R, Wang T, et al.A bivalent β-carboline derivative inhibits macropinocytosis-dependent entry of pseudorabies virus by targeting the kinase DYRK1A.Journal of Biological Chemistry.2023: 104605. 3. Wang K, Ge Y, Yang Y, et al.Vascular endothelial cellular mechanics under hyperglycemia and its role in tissue regeneration.Regenerative Biomaterials.2024: rbae004.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Cancer Active Compound Library Kinase Inhibitor Library GPCR Compound Library Anti-Obesity Compound Library Anti-Pancreatic Cancer Compound Library Anti-Cancer Metabolism Compound Library Anti-Cancer Compound Library Anti-Aging Compound Library Anti-Ovarian Cancer Compound Library

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Keywords

ML141 71203-35-5 Apoptosis Cell Cycle/Checkpoint GPCR/G Protein MAPK Ras CDK inhibit toxicity cells CID-2950007 ML 141 ovarian noncompetitive CID 2950007 acute ML-141 anxiety allosteric CID2950007 cancer OVCA429 Inhibitor inhibitor

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