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MG-132

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Catalog No. T2154Cas No. 133407-82-6
Alias Z-LLL-al, Z-Leu-Leu-Leu-CHO, MG132

MG-132 (Z-Leu-Leu-Leu-al) is a 26S proteasome inhibitor (IC50=100 nM) that is cell-permeable and reversible. MG-132 acts as an autophagy activator and also induces apoptosis.

MG-132
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MG-132

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Purity: 99.99%
Catalog No. T2154Alias Z-LLL-al, Z-Leu-Leu-Leu-CHO, MG132Cas No. 133407-82-6
MG-132 (Z-Leu-Leu-Leu-al) is a 26S proteasome inhibitor (IC50=100 nM) that is cell-permeable and reversible. MG-132 acts as an autophagy activator and also induces apoptosis.
Pack SizePriceUSA WarehouseGlobal WarehouseQuantity
10 mg$40In StockIn Stock
25 mg$81In StockIn Stock
50 mg$147In StockIn Stock
100 mg$228In StockIn Stock
200 mg$396In StockIn Stock
500 mg$497In StockIn Stock
1 mL x 10 mM (in DMSO)$39In StockIn Stock
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Purity:99.99%
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COA HNMR HPLC MS

Product Introduction

Bioactivity
Description
MG-132 (Z-Leu-Leu-Leu-al) is a 26S proteasome inhibitor (IC50=100 nM) that is cell-permeable and reversible. MG-132 acts as an autophagy activator and also induces apoptosis.
Targets&IC50
Calpain:1.2 μM (cell free), COS-7 cells:< 10 μM, 20S proteasome:100 nM (cell free), HCT116 cells:0.82 μM
In vitro
METHODS: Human cervical cancer cells HeLa were treated with MG-132 (0.5-30 μM) for 24 h, and cell growth inhibition was detected by MTT.
RESULTS: MG-132 dose-dependently inhibited HeLa cell growth with an IC50 of approximately 5 μM. [1]
METHODS: Human mesothelioma cells NCI-H2452 were treated with MG-132 (0.25-2 μM) for 36 h, and the expression levels of target proteins were detected by Western Blot.
RESULTS: MG-132 treatment induces cleavage of caspases 3 and 7, Bid, and PARP in NCI-H2452 cells. MG-132 induces a caspase-dependent apoptosis. [2]
METHODS: Human melanoma cells MeWo were treated with MG-132 (0.01-1 μM) for 24 h, and the cell cycle profiles were analyzed by Flow Cytometry.
RESULTS: MG-132 induces cell cycle arrest at G2 phase in MeWo cells. [3]
In vivo
METHODS: To detect anti-tumor activity in vivo, MG-132 (1 mg/kg) was injected intravenously into C.B-17/lcr-scid/scidJcl mice harboring the human cervical cancer tumors HeLa, CaSki, or C33A twice a week for 4 weeks.
RESULTS: MG-132 significantly inhibited the growth of human cervical cancer tumors, indicating antitumor activity in vivo. [4]
METHODS: To investigate the effects of long-term treatment with MG-132 on cardiac hypertrophy and its associated molecular mechanisms, MG-132 (0.1 mg/kg) was injected intraperitoneally into rats with an abdominal aortic band (AAB) once daily for 8 weeks.
RESULTS: MG-132 treatment significantly attenuated left ventricular myocyte area, left ventricular weight/body weight, and lung weight/body weight ratios, decreased left ventricular diastolic diameter and wall thickness, and increased the shortening fraction in AAB rats. MG-132 treatment significantly reversed the elevated levels of ERK1/2 and JNK1 phosphorylation in AAB rats. [5]
SynonymsZ-LLL-al, Z-Leu-Leu-Leu-CHO, MG132
Chemical Properties
Molecular Weight475.62
FormulaC26H41N3O5
Cas No.133407-82-6
SmilesCC(C)C[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)OCc1ccccc1)C=O
Relative Density.1.073 g/cm3
Storage & Solubility Information
Storagestore at low temperature | store at 4°C | Shipping with blue ice/Shipping at ambient temperature.
Solubility Information
H2O: Insoluble
Ethanol: 47.5 mg/mL (99.87 mM), Sonication is recommended.
DMSO: 240 mg/mL (504.6 mM), Sonication is recommended.
In Vivo Formulation
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 9 mg/mL (18.92 mM), Suspension.
Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM2.1025 mL10.5126 mL21.0252 mL105.1259 mL
5 mM0.4205 mL2.1025 mL4.2050 mL21.0252 mL
10 mM0.2103 mL1.0513 mL2.1025 mL10.5126 mL
20 mM0.1051 mL0.5256 mL1.0513 mL5.2563 mL
50 mM0.0421 mL0.2103 mL0.4205 mL2.1025 mL
DMSO
1mg5mg10mg50mg
100 mM0.0210 mL0.1051 mL0.2103 mL1.0513 mL

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TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH2O. So your working solution concentration is 2 mg/mL。
Stock solution preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (stock solution concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH2OTargetMol | reagent mix well and clarify
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2 Enter the in vivo formulation:
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Keywords

proteolyticProteasomepeptideMG-132MG 132InhibitorinhibitcomplexcalpainAutophagyApoptosisaldehyde26S20S proteasome
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