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KN-62

Catalog No. T2694 CAS 127191-97-3

Synonyms: KN 62, KN62

KN-62 is a potent and specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with Ki of 0.9 μM.

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KN-62, CAS 127191-97-3

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	KN-62 is a potent and specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with Ki of 0.9 μM.
Targets&IC50	CaMKⅤ:0.8 μM(Ki), CaMKII:0.9 μM(Ki)
Kinase Assay	Kinase assay: Total kinase activity of CaMKII, determined in a standard 2 min assay (100 μL), contained 35 mM HEPES, 10 mM MgCl2, 1 mM CaCl2, 10 μg of chicken gizzard myosin 20-kD light chain, 0.1 μM calmodulin, and 10 μM [γ-33]ATP at 30 °C. The kinase reaction is halted by adding 1 mL of 10% trichloroacetic acid.
Cell Research	For cell growth analysis, K562 cells are plated in a 3-cm dish with 5 mL of culture medium containing various concentration of KN-62. After two days in these condition cell numbers are counted. (Only for Reference)

Synonyms	KN 62, KN62
Molecular Weight	721.85
Formula	C38H35N5O6S2
CAS No.	127191-97-3

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 72.2 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Tokumitsu H, et al. J Biol Chem. 1990, 265(8), 4315-4320. 2. Wenham RM, et al. Biochem Biophys Res Commun. 1992, 189(1), 128-133. 3. Minami H, et al. Biochem Biophys Res Commun. 1994, 199(1), 241-248. 4. Murray KD, et al. Eur J Neurosci. 1998, 10(1), 377-387. 5. Pellicena P, et al. Front Pharmacol. 2014, 20;5:21. 6. Manosso LM, et al. Antidepressant-like effect of zinc is dependent on signaling pathways implicated in BDNF modulation. Prog Neuropsychopharmacol Biol Psychiatry. 2015 Jun 3;59:59-67.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Autophagy Compound Library Anti-Obesity Compound Library Inhibitor Library Ion Channel Inhibitor Library Covalent Inhibitor Library Kinase Inhibitor Library Bioactive Lipid Compound Library Neuronal Signaling Compound Library Bioactive Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

KN-62 127191-97-3 离子通道神经科学自噬 Autophagy CaMK P2X Receptor noncompetitive antagonism KN 62 inhibit P2XRs Calmodulin-dependent protein kinases HEK293 cells Calmodulin-dependent kinases Inhibitor autophosphorylation KN62 inhibitor

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