Powder: -20°C for 3 years | In solvent: -80°C for 1 year
KN-62 is a potent and specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with Ki of 0.9 μM.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 30.00 | |
2 mg | In stock | $ 44.00 | |
5 mg | In stock | $ 68.00 | |
10 mg | In stock | $ 113.00 | |
25 mg | In stock | $ 229.00 | |
50 mg | In stock | $ 448.00 | |
100 mg | In stock | $ 658.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 113.00 |
Description | KN-62 is a potent and specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with Ki of 0.9 μM. |
Targets&IC50 | CaMK V:0.8 μM(Ki), CaMK II:0.9 μM(Ki) |
In vitro | KN-62 administration in adult rats reduces the expression levels of brain-derived neurotrophic factor (BDNF) mRNA induced by epilepsy in the brain. |
In vivo | KN-62 inhibits the proliferation of K562 cells and blocks cell cycle progression. When administered at 10 μM to rat pancreatic islet cells, KN-62 inhibits insulin secretion stimulated by carbachol and potassium. Furthermore, KN-62 suppresses the phosphorylation of Ca2+/CaM kinase induced by A23187 in PC12 D cells. |
Kinase Assay | Kinase assay: Total kinase activity of CaMKII, determined in a standard 2 min assay (100 μL), contained 35 mM HEPES, 10 mM MgCl2, 1 mM CaCl2, 10 μg of chicken gizzard myosin 20-kD light chain, 0.1 μM calmodulin, and 10 μM [γ-33]ATP at 30 °C. The kinase reaction is halted by adding 1 mL of 10% trichloroacetic acid. |
Cell Research | For cell growth analysis, K562 cells are plated in a 3-cm dish with 5 mL of culture medium containing various concentration of KN-62. After two days in these condition cell numbers are counted. (Only for Reference) |
Molecular Weight | 721.84 |
Formula | C38H35N5O6S2 |
CAS No. | 127191-97-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 72.2 mg/mL (100 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
KN-62 127191-97-3 Autophagy Membrane transporter/Ion channel Neuroscience P2X Receptor CaMK noncompetitive antagonism inhibit KN62 P2XRs Calmodulin-dependent protein kinases HEK293 cells KN 62 Calmodulin-dependent kinases Inhibitor autophosphorylation inhibitor