Powder: -20°C for 3 years
In solvent: -80°C for 2 years
KN-62 is a potent and specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with Ki of 0.9 μM.
Description | KN-62 is a potent and specific Ca2+/calmodulin-dependent protein kinase II (CaMKII) inhibitor with Ki of 0.9 μM. |
Targets&IC50 | CaMKⅤ:0.8 μM(Ki), CaMKII:0.9 μM(Ki) |
Kinase Assay | Kinase assay: Total kinase activity of CaMKII, determined in a standard 2 min assay (100 μL), contained 35 mM HEPES, 10 mM MgCl2, 1 mM CaCl2, 10 μg of chicken gizzard myosin 20-kD light chain, 0.1 μM calmodulin, and 10 μM [γ-33]ATP at 30 °C. The kinase reaction is halted by adding 1 mL of 10% trichloroacetic acid. |
Cell Research | For cell growth analysis, K562 cells are plated in a 3-cm dish with 5 mL of culture medium containing various concentration of KN-62. After two days in these condition cell numbers are counted. (Only for Reference) |
Synonyms | KN 62, KN62 |
Molecular Weight | 721.85 |
Formula | C38H35N5O6S2 |
CAS No. | 127191-97-3 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 72.2 mg/mL (100 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
KN-62 127191-97-3 离子通道 神经科学 自噬 Autophagy CaMK P2X Receptor noncompetitive antagonism KN 62 inhibit P2XRs Calmodulin-dependent protein kinases HEK293 cells Calmodulin-dependent kinases Inhibitor autophosphorylation KN62 inhibitor