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Anti-Phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CaMK2 alpha (Tyr230). Anti-Phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $528 | 7-10 days |
Description | Anti-Phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CaMK2 alpha (Tyr230). Anti-Phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM. |
Ig Type | IgG |
Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Sample: Cerebrum (Rat) cell Lysate at 40 μg Primary: Anti-phospho-CaMK2 alpha (Tyr230) (TMAB-01398) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 54 kDa 5. Blank control: K562. Primary Antibody (green line): Rabbit Anti-phospho-CaMK2 alpha (Tyr230) antibody (TMAB-01398) Dilution: 2 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. 6. Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 7. Sample: Lane 1: Mouse Muscle tissue lysates Lane 2: Mouse Heart tissue lysates Primary: Anti-phospho-CaMK2 alpha (Tyr230) (TMAB-01398) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kDa Observed band size: 50 kDa |
Application | WB,IHC-P,IHC-Fr,IF,FCM |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse. Note=Postsynaptic lipid rafts. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Immunogen | KLH conjugated Synthesised phosphopeptide: human CaMK2 alpha around the phosphorylation site of Tyr231 |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Calmodulin / CaMK,Hypoxia,Hypertrophy,Response to hypoxia |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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