Anti-Phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CaMK2 alpha (Tyr230). Anti-Phospho-CaMK2 alpha (Tyr230) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Human gastric); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha (Tyr230)) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Sample:
Cerebrum (Rat) cell Lysate at 40 μg
Primary: Anti-phospho-CaMK2 alpha (Tyr230) (TMAB-01398) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
5. Blank control: K562. Primary Antibody (green line): Rabbit Anti-phospho-CaMK2 alpha (Tyr230) antibody (TMAB-01398) Dilution: 2 μg/Test; Secondary Antibody: Goat anti-rabbit IgG-FITC Dilution: 0.5 μg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
6. Paraformaldehyde-fixed, paraffin embedded (Mouse stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (p-CaMK2 alpha) Polyclonal Antibody, Unconjugated (TMAB-01398) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
7. Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Mouse Heart tissue lysates
Primary: Anti-phospho-CaMK2 alpha (Tyr230) (TMAB-01398) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2ug/Test

Polyclonal

KLH conjugated Synthesised phosphopeptide: human CaMK2 alpha around the phosphorylation site of Tyr231

Human

Calmodulin / CaMK,Hypoxia,Hypertrophy,Response to hypoxia