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Anti-CAMK2 alpha Polyclonal Antibody 2

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Catalog No. TMAB-00275
Alias KCC2A, CaMK-II subunit alpha, CaMKII alpha, CaMKII, CAMKB, CAMKA, CAMK2α, CAMK2D, CAMK2B, CAMK2A, CAMK2 α, CaMK II delta subunit, CaMK II beta subunit, CaMK II alpha subunit, CaMK 2a, CAM2, CaM kinase II subunit alpha, CaM kinase II delta subunit, CaM kinase II delta chain, CaM kinase II beta subunit, CaM kinase II beta chain, CaM kinase II alpha subunit, CaM kinase II alpha chain, Calcium/calmodulin-dependent protein kinase(CaM kinase) II alpha, Calcium/calmodulin-dependent protein kinase type IIA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, Calcium/Calmodulin Dependent Protein Kinase II G, Calcium/calmodulin dependent protein kinase II alpha, Calcium calmodulin dependent protein kinase II alpha-B subunit, Calcium calmodulin dependent protein kinase II, Calcium calmodulin dependent protein kinase CaM kinase II alpha, alpha CaMKII

Anti-CAMK2 alpha Polyclonal Antibody 2 is a Rabbit antibody targeting CAMK2 alpha. Anti-CAMK2 alpha Polyclonal Antibody 2 can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.

Anti-CAMK2 alpha Polyclonal Antibody 2

Anti-CAMK2 alpha Polyclonal Antibody 2

😃Good
Catalog No. TMAB-00275Alias KCC2A, CaMK-II subunit alpha, CaMKII alpha, CaMKII, CAMKB, CAMKA, CAMK2α, CAMK2D, CAMK2B, CAMK2A, CAMK2 α, CaMK II delta subunit, CaMK II beta subunit, CaMK II alpha subunit, CaMK 2a, CAM2, CaM kinase II subunit alpha, CaM kinase II delta subunit, CaM kinase II delta chain, CaM kinase II beta subunit, CaM kinase II beta chain, CaM kinase II alpha subunit, CaM kinase II alpha chain, Calcium/calmodulin-dependent protein kinase(CaM kinase) II alpha, Calcium/calmodulin-dependent protein kinase type IIA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, Calcium/Calmodulin Dependent Protein Kinase II G, Calcium/calmodulin dependent protein kinase II alpha, Calcium calmodulin dependent protein kinase II alpha-B subunit, Calcium calmodulin dependent protein kinase II, Calcium calmodulin dependent protein kinase CaM kinase II alpha, alpha CaMKII
Anti-CAMK2 alpha Polyclonal Antibody 2 is a Rabbit antibody targeting CAMK2 alpha. Anti-CAMK2 alpha Polyclonal Antibody 2 can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3737-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-CAMK2 alpha Polyclonal Antibody 2 is a Rabbit antibody targeting CAMK2 alpha. Anti-CAMK2 alpha Polyclonal Antibody 2 can be used in ICC/IF,IF,IHC-Fr,IHC-P,WB.
SynonymsKCC2A, CaMK-II subunit alpha, CaMKII alpha, CaMKII, CAMKB, CAMKA, CAMK2α, CAMK2D, CAMK2B, CAMK2A, CAMK2 α, CaMK II delta subunit, CaMK II beta subunit, CaMK II alpha subunit, CaMK 2a, CAM2, CaM kinase II subunit alpha, CaM kinase II delta subunit, CaM kinase II delta chain, CaM kinase II beta subunit, CaM kinase II beta chain, CaM kinase II alpha subunit, CaM kinase II alpha chain, Calcium/calmodulin-dependent protein kinase(CaM kinase) II alpha, Calcium/calmodulin-dependent protein kinase type IIA, Calcium/calmodulin-dependent protein kinase type II subunit alpha, Calcium/Calmodulin Dependent Protein Kinase II G, Calcium/calmodulin dependent protein kinase II alpha, Calcium calmodulin dependent protein kinase II alpha-B subunit, Calcium calmodulin dependent protein kinase II, Calcium calmodulin dependent protein kinase CaM kinase II alpha, alpha CaMKII
Ig Type
IgG
Reactivity
Human,Mouse,Rat
Verified Activity
1. Sample:
Lane 1: Mouse Heart tissue lysates
Lane 2: Rat Heart tissue lysates
Primary: Anti-CaMK2 alpha (TMAB-00275) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa
2. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMK2 alpha) Polyclonal Antibody, Unconjugated (TMAB-00275) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. 25 μg total protein per Lane of various lysates probed with CaMK2 alpha polyclonal antibody, unconjugated (TMAB-00275) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min.
4. 4% Paraformaldehyde-fixed SH-SY5Y (H) cell; Triton X-100 at RT for 20 min; Antibody incubation with (CaMK2 alpha) polyclonal Antibody, unconjugated (TMAB-00275) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nucleus. PBS instead of the primary antibody was used as the blank control.
Application
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell junction, synapse, presynaptic cell membrane. Cell junction, synapse. Note=Postsynaptic lipid rafts.
Tissue SpecificityWidely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.
ConstructionPolyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Concentration1 mg/mL
Research BackgroundThe product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008].
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human CaMK2 alpha
Antigen Species
Human
Gene Name
CAMK2A
Gene ID
Protein Name
Calcium/calmodulin-dependent protein kinase type II subunit alpha
Uniprot ID
Biology Area
Calmodulin / CaMK,Hypoxia,Hypertrophy,Response to hypoxia
Function
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
Chemical Properties
Molecular WeightTheoretical: 54 kDa.
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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