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Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody
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Catalog No. TMAB-01399
Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CaMKII (Thr287). Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody
Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody
😃Good
Catalog No. TMAB-01399
Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CaMKII (Thr287). Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody is a Rabbit antibody targeting Phospho-CaMKII (Thr287). Anti-Phospho-CaMKII (Thr287) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Chicken,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (CaMKII (Thr286)) Polyclonal Antibody, Unconjugated (TMAB-01399) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | IHC-P,IHC-Fr,IF |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse. Note=Postsynaptic lipid rafts. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a beta chain. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human CaMK2 alpha around the phosphorylation site of Thr287 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Calmodulin / CaMK,Hypoxia,Hypertrophy,Response to hypoxia |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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