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Indoximod

Catalog No. T6543 CAS 110117-83-4

Synonyms: 1-Methyl-D-tryptophan, Indoximod (NLG-8189）, NLG-8189

Indoximod is a methylated tryptophan with immune checkpoint inhibitory activity. Indoximod inhibits the enzyme indoleamine 2, 3-dioxygenase (<a href="/target/IDO" style="display: inline; color: #c13a36">IDO</a>), which degrades the essential amino acid tryptophan, and may increase or maintain tryptophan levels important to T cell function. Tryptophan depletion is associated with immunosuppression involving T cell arrest and anergy.

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Indoximod, CAS 110117-83-4

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Purity: 98.49%

Purity: 99.5%

Purity: 99.15%

Purity: 98.68%

Purity: 97.22%

Purity: 98%

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Storage & Solubility Information

Description	Indoximod is a methylated tryptophan with immune checkpoint inhibitory activity. Indoximod inhibits the enzyme indoleamine 2, 3-dioxygenase (IDO), which degrades the essential amino acid tryptophan, and may increase or maintain tryptophan levels important to T cell function. Tryptophan depletion is associated with immunosuppression involving T cell arrest and anergy.
Targets&IC50	IDO:19 μM(Ki)
In vitro	Indoximod significantly reverses the suppression of T cells created by IDO-expressing dendritic cells, using both human monocyte-derived dendritic cells and murine dendritic cells isolated directly from tumor-draining lymph nodes. [1] Indoximod activates a Trp sufficiency signal that stimulates mTOR, and relieves IDO-induced Trp deprivation as well as an autophagic response. [2]
In vivo	In mouse models of transplantable melanoma and transplantable and autochthonous breast cancer, Indoximod (400 mg/kg p.o.) enhances antitumor immunity in the setting of combined chemo-immunotherapy regimens. [1]
Kinase Assay	1MT enantiomers are solubilized in DMSO containing 0.1N HCl and added at concentrations of 100, 50, and 0 μM to wells containing the reaction mixture with tryptophan (0-200 μM) followed by addition of IDO enzyme. Plates are sealed and incubated 1 hr in a humidified 37°C incubator, after which the reactions are terminated by addition of 12.5 μl 30% TCA per well. Plates are then resealed in plastic wrap, incubated 30 min at 50°C to hydrolyze the reaction product N-formylkynurenine to kynurenine, and centrifuged. Supernatants are transferred to a flat-bottom 96-well plate, mixed with 100 μl Ehrlich reagent and incubated 10 min at room temperature. Absorbance at 490 nm is read[1].

Synonyms	1-Methyl-D-tryptophan, Indoximod (NLG-8189）, NLG-8189
Molecular Weight	218.256
Formula	C12H14N2O2
CAS No.	110117-83-4

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 10mM

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Hou DY, et al. Cancer Res. 2007, 67(2), 792-801. 2. Metz R, et al. Oncoimmunology. 2012, 1(9), 1460-1468. 3. Nguyen D J M, Theodoropoulos G, Li Y Y, et al. Targeting the kynurenine pathway for the treatment of cisplatin resistant lung cancer[J]. Molecular Cancer Research. 2019: molcanres. 0239.2019. 4. Nguyen D J M, Theodoropoulos G, Li Y Y, et al. Targeting the kynurenine pathway for the treatment of cisplatin-resistant lung cancer[J]. Molecular Cancer Research. 2020, 18(1): 105-117.

Citations

1. Nguyen D J M, Theodoropoulos G, Li Y Y, et al. Targeting the kynurenine pathway for the treatment of cisplatin resistant lung cancer. Molecular Cancer Research. 2019: molcanres. 0239.2019

Related compound libraries

This product is contained In the following compound libraries：

Pediatric Drug Library ReFRAME Related Library Anti-Lung Cancer Compound Library Metabolism Compound Library Orally Active Compound Library Anti-Metabolism Disease Compound Library Anti-Cancer Metabolism Compound Library Autophagy Compound Library Immunology/Inflammation Compound Library Drug Repurposing Compound Library

Related Products

Related compounds with same targets

BMS-986242 IDO inhibitor 1 IDO-IN-1 Indoximod Palmatine Coptisine sulfate PCC0208009 (S)-Indoximod

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Keywords

Indoximod 110117-83-4 Metabolism Indoleamine 2,3-Dioxygenase (IDO) mTORC1 NLG8189 inhibit 1-Methyl-D-tryptophan tryptophan Inhibitor Indoximod (NLG-8189） Trp mimetic cancer NLG-8189 NLG 8189 immunotherapeutic vaccines inhibitor

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