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INH1

Catalog No. T2502 CAS 313553-47-8

Synonyms: IBT13131

INH1 (IBT13131) is a cell-permeable Hec1 inhibitor that specifically disrupts the Hec1/Nek2 interaction.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

INH1, CAS 313553-47-8

Pack Size	Availability	Price/USD
10 mg	In stock	$ 39.00
25 mg	In stock	$ 81.00
50 mg	In stock	$ 147.00
100 mg	In stock	$ 239.00
1 mL * 10 mM (in DMSO)	In stock	$ 29.00

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Biological Description

Chemical Properties

Storage & Solubility Information

Description	INH1 (IBT13131) is a cell-permeable Hec1 inhibitor that specifically disrupts the Hec1/Nek2 interaction.
Targets&IC50	Hec1:10-21 μM
In vitro	Intraperitoneal injection of 100 mg/kg INH1 inhibits the growth of mammary tumors in mice with MDA-MB-468 human breast cancer xenografts.
In vivo	INH1 effectively inhibits the proliferation of human breast cancer cells with a GI50 of 10-21 μM. Additionally, INH1 induces cell-killing activity by disrupting the spindle checkpoint-regulated Hec1/Nek2 pathway.
Kinase Assay	Binding assays: Surface plasma resonance (SPR) assays are performed at 22.5°C in HBSD buffer [10 mmol/L HEPES, 150 mmol/L NaCl, 0.1% DMSO (pH 7.5)] on Biacore 3000. 6×His-Hec1 and GST-Nek2 are purified. NTA sensor chip or glutathione-modified CM5 chip are used to capture His-Hec1 and GST-Nek2, respectively. The capture level is about 140 to 180 resonance units (RU) at the flow rate of 5 μL/min. For the binding assay, chips are sequentially treated with compounds (1 or 20 μmol/L) and then proteins (50 μg/mL). Retained RUs are recorded and processed (triplicate experiments).
Cell Research	Standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays with a 3-d drug treatment procedure are performed to measure the dose-dependent cytotoxicity of INH1 in cultured cells. Triplicate sets are measured and compiled for final data presentation.(Only for Reference)

Synonyms	IBT13131
Molecular Weight	308.4
Formula	C18H16N2OS
CAS No.	313553-47-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 30.8 mg/mL (100 mM)

Ethanol: 3.1 mg/mL (10 mM)

References and Literature

1. Wu G, et al. Cancer Res. 2008, 68(20), 8393-8399.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Microtubule-Targeted Compound Library Anti-Cancer Active Compound Library Anti-Cancer Compound Library PPI Inhibitor Library Bioactive Compound Library Apoptosis Compound Library Cytoskeletal Signaling Pathway Compound Library NO PAINS Compound Library Bioactive Compounds Library Max

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

INH1 313553-47-8 Apoptosis Cytoskeletal Signaling Microtubule Associated inhibit INH 1 Inhibitor IBT-13131 INH-1 IBT13131 IBT 13131 inhibitor

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