Home Tools

Hesperadin

Catalog No. T6532 CAS 422513-13-1

Hesperadin(IC50=250 nM) effectively inhibits Aurora B. It potently reduces the activity of AMPK, MAPKAP-K1, MKK1, Lck, CHK1 and PHK, but it could not inhibit MKK1 activity in vivo.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Hesperadin, CAS 422513-13-1

Pack Size	Availability	Price/USD
2 mg	In stock	$ 33.00
5 mg	In stock	$ 52.00
10 mg	In stock	$ 77.00
25 mg	In stock	$ 128.00
50 mg	In stock	$ 207.00
1 mL * 10 mM (in DMSO)	In stock	$ 59.00

Bulk Inquiry

Select Batch

Purity: 99.44%

Purity: 98.04%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Hesperadin(IC50=250 nM) effectively inhibits Aurora B. It potently reduces the activity of AMPK, MAPKAP-K1, MKK1, Lck, CHK1 and PHK, but it could not inhibit MKK1 activity in vivo.
Targets&IC50	Aurora B:250 nM
In vitro	In HeLa cells, Hesperadin causing defects in mitosis and cytoplasmic division, leading to cell proliferation and polyploidization stopped, because of Aurora B function inhibition during chromosome connection. Addition of 20-100 nM Hesperadin leading to loss of phosphorylation of the mitogenic histone H3 at the Ser10 site. When Hesperadin concentration was 1 μM, other kinases (AMPK, Lck, MKK1, MAPKAP-K1, CHK1, and PHK) activity were significantly reduced.In an in vitro kinase assay, Hesperadin (IC50 = 40 nM) blocked recombinant trypton histone H3 phosphorylation by T. brucei Aurora kinase-1 (TbAUK1) from the pathogenic Trypanosoma brucei. Hesperadin (IC50 = 48 nM) significantly inhibited the growth of cultured infectious blood form (BF) cells, while Hesperadin (IC50 = 550 nM) and weakly inhibited the growth of insect circulation stage (PF) cells.
Kinase Assay	For the Aurora B kinase assay, HeLa cells are lysed in a buffer containing 50 mM NaCl, then centrifuging at 13,000 rpm for 20 minutes at 4 °C. Discard supernatant, add 15 mL lysis buffer containing 250 mM NaCl in order to obtain active Aurora B kinase. Centrifuging at low-speed supernatant of the latter extract is used for immunoprecipitation. Monoclonal mouse anti–AIM-1, or mouse anti-HA, is coupled to GammaBind Plus Sepharose, and beads are rotated over-end in the extract for 90 minutes at 4 °C. Beads are washed, aliquoted, and washed in kinase buffer (20 mM Tris, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10 mM NaF). The kinase assay is performed with 10 μL beads in 20 μL kinase buffer containing 5 μg histone H3, 10 μM ATP, 2.5 μCi [γ-32P]ATP, and different concentrations of Hesperadin for 20 minutes at 37 °C.
Cell Research	HeLa cells and PtK1 cells are added Hesperadin 500 nM for 24 and 48 hours.

Molecular Weight	516.65
Formula	C29H32N4O3S
CAS No.	422513-13-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 55.3 mg/mL (100 mM)

Ethanol: 27.7 mg/mL (50 mM)

References and Literature

1. Hauf S, et al. J Cell Biol, 2003, 161(2), 281-294. 2. Jetton N, et al. Mol Microbiol, 2009, 72(2), 442-458. 3. Wang J, Yan X, Chen H, et al. Enhanced UV-B radiation affects AUR1 regulation of mitotic spindle morphology leading to aberrant mitosis[J]. Plant Physiology and Biochemistry. 159: 160-170.

Citations

1. Wang J, Yan X, Chen H, et al. Enhanced UV-B radiation affects AUR1 regulation of mitotic spindle morphology leading to aberrant mitosis. Plant Physiology and Biochemistry. 159: 160-170.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Kinase Inhibitor Library Anti-Parasitic Compound Library Epigenetics Compound Library Cell Cycle Compound Library Autophagy Compound Library Anti-Viral Compound Library Bioactive Compounds Library Max Anti-Aging Compound Library Anti-Infection Compound Library

Related Products

Related compounds with same targets

zapnometinib Camptothecin Vadimezan Oseltamivir acid Harringtonine Curcumin Oplodiol Citrullinated LL-37 3cit

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Hesperadin 422513-13-1 Autophagy Cell Cycle/Checkpoint Chromatin/Epigenetic Microbiology/Virology Influenza Virus Parasite Aurora Kinase Aurora procyclic forms bloodstream inhibit Inhibitor inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.