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Guanosine

Catalog No. T6523 CAS 118-00-3

Synonyms: DL-Guanosine, Vernine, Guanozin

Guanosine (DL-Guanosine) is a purine nucleoside formed from a beta-N9-glycosidic bond between guanine and a ribose ring and is essential for metabolism.

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Guanosine, CAS 118-00-3

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2 g	In stock	$ 30.00
1 mL * 10 mM (in DMSO)	In stock	$ 30.00

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Purity: 99.76%

Purity: 99.31%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Guanosine (DL-Guanosine) is a purine nucleoside formed from a beta-N9-glycosidic bond between guanine and a ribose ring and is essential for metabolism.
In vitro	Guanosine is a purine nucleoside comprising guanine attached to a ribose (ribofuranose) ring via a β-N9-glycosidic bond. Guanosine can be phosphorylated to become guanosine monophosphate (GMP), cyclic guanosine monophosphate (cGMP), guanosine diphosp
Kinase Assay	Tyrosine kinase assays: The tyrosine kinase activities are determined in 96-well ELISA plates precoated with 20 μg/mL Poly (Glu,Tyr)4:1. First, 80 μL of 5 μM ATP solution diluted in kinase reaction buffer (50 mM HEPES pH 7.4, 20 mM MgCl2, 0.1 mM MnCl2, 0.2 mM Na3VO4, 1 mM DTT) is added to each well. Various concentrations of AST-1306 diluted in 10 μL of 1% DMSO (v/v) are then added to each reaction well, with 1% DMSO (v/v) used as the negative control. Subsequently, the kinase reaction is initiated by the addition of purified tyrosine kinase proteins diluted in 10 μL of kinase reaction buffer solution. Experiments at each concentration are performed in duplicate. After incubation for 60 min at 37 °C, the plate is washed three times with phosphate buffered saline (PBS) containing 0.1% Tween 20 (T-PBS). Next, 100 μL anti-phosphotyrosine antibody (PY99, 1:500 dilution) diluted in T-PBS containing 5 mg/mL BSA is added. After 30 min incubation at 37 °C, the plate is washed three times as before. Horseradish peroxidase-conjugated goat anti-mouse IgG (100 μL) diluted 1:2000 in T-PBS containing 5 mg/mL BSA is added. The plate is reincubated at 37 °C for 30 min, and then washed with PBS. Finally, 100 μL of a solution containing 0.03 % Water2 and 2 mg/mL o-phenylenediamine in 0.1 M citrate buffer, pH 5.5, is added and samples are incubated at room temperature until color emerged. The reaction is terminated by the addition of 50 μL of 2 M H2SO4, and the plate is read using a multi-well spectrophotometer at 490 nm. The inhibition rate (%) is calculated using the following equation: [1-(A490 treated /A490 control)] ×100%. IC50 values are determined from the results of at least three independent tests and calculated by Logit method.
Source	Natural Products > Lythraceae > Punica

Synonyms	DL-Guanosine, Vernine, Guanozin
Molecular Weight	283.24
Formula	C10H13N5O5
CAS No.	118-00-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 52 mg/mL (183.6 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Sáenz JB, et al. Nat Chem Biol. 2009, 5(3), 157-16

Citations

1. Chen J, Li T, Huang D, et al.Integrating UHPLC-MS/MS quantitative analysis and exogenous purine supplementation to elucidate the antidepressant mechanism of Chaigui granules by regulating purine metabolism.Journal of Pharmaceutical Analysis.2023

Related compound libraries

This product is contained In the following compound libraries：

Miao medicine Compound Library Human Endogenous Metabolite Library Anti-Tumor Natural Product Library RO5 Drug-like Natural Product Library Gut Microbial Metabolite Library Nucleotide Compound Library Human Endogenous Metabolite Compound Library Plus Bioactive Compounds Library Max NO PAINS Compound Library Anti-COVID-19 Compound Library

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Keywords

Guanosine 118-00-3 Metabolism Microbiology/Virology HSV Endogenous Metabolite Inhibitor DL-Guanosine Herpes simplex virus Vernine Guanozin inhibit inhibitor

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