Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Fasudil hydrochloride (HA-1077) is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
50 mg | In stock | $ 35.00 | |
100 mg | In stock | $ 45.00 | |
200 mg | In stock | $ 68.00 | |
500 mg | In stock | $ 97.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 50.00 |
Description | Fasudil hydrochloride (HA-1077) is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively. |
Targets&IC50 | ROCK1:0.33 μM(ki), PKC:9.3 μM(ki), PKA:1.0 μM(ki), MLCK:55 μM(ki) |
Kinase Assay | Cyclic AMP-dependent protein kinase activity is assayed in a reaction mixture containing, in a final volume of 0.2 mL, 50 mM Tris-HCl (pH 7.0), 10 mM magnesium acetate, 2 mM EGTA, 1 μM cyclic AMP or absence of cyclic AMP, 3.3 to 20 μM [r-32P] ATP (4×105 c.p.m.), 0.5 μg of the enzyme, 100 μg of histone H2B and compound. The mixture is incubated at 30°C for 5 min. The reaction is terminated by adding 1mL of ice-cold 20% trichloroacetic acid after adding 500 μg of bovine serum albumin as a carrier protein. The sample is centrifuged at 3000 r.p.m. for 15min, the pellet is resuspended in ice-cold 10% trichloro-acetic acid solution and the centrifugation-resuspension cycle is repeated three times. The final pellet is dissolved in 1 mL of 1 N NaOH and radioactivity is measured with a liquid scintillation counter. |
Synonyms | Fasudil (HA-1077) HCl, HA-1077, Fasudil HCl, HA-1077 hydrochloride, AT-877 |
Molecular Weight | 327.83 |
Formula | C14H18ClN3O2S |
CAS No. | 105628-07-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 32.8 mg/mL (100 mM)
DMSO: 24.6 mg/mL (75 mM)
You can also refer to dose conversion for different animals. More
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Fasudil hydrochloride 105628-07-7 Autophagy Cell Cycle/Checkpoint Chromatin/Epigenetic Cytoskeletal Signaling Membrane transporter/Ion channel Metabolism Microbiology/Virology Proteases/Proteasome Stem Cells Tyrosine Kinase/Adaptors Calcium Channel ROCK Serine/threonin kinase HIV Protease PKA PKC TWNT-4 cells TIMP-1 HA 1077 a-SMA Protein kinase A Ca channels HIV rat HSCs AT877 Rho-associated protein kinase human HSC ERK1/2 Rho-kinase inhibit Ca2+ channels Rho-associated kinase HA1077 Hydrochloride Fasudil (HA-1077) HCl p38 lysophoaphatidic acid Fasudil Ca2+ channel antagonist JNK HA 1077 Hydrochloride HA-1077 protein kinases collagen LPA HA1077 Inhibitor Fasudil HCl Fasudil Hydrochloride Protein kinase C vasodilator ROK HA-1077 hydrochloride AT 877 Human immunodeficiency virus orally active AT-877 HA-1077 Hydrochloride inhibitor