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Fasudil hydrochloride

Catalog No. T3060   CAS 105628-07-7
Synonyms: Fasudil (HA-1077) HCl, HA-1077, Fasudil HCl, HA-1077 hydrochloride, AT-877

Fasudil hydrochloride (HA-1077) is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively.

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Fasudil hydrochloride Chemical Structure
Fasudil hydrochloride, CAS 105628-07-7
Pack Size Availability Price/USD Quantity
50 mg In stock $ 35.00
100 mg In stock $ 45.00
200 mg In stock $ 68.00
500 mg In stock $ 97.00
1 mL * 10 mM (in DMSO) In stock $ 50.00
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Purity: 100%
Purity: 99.98%
Purity: 99.54%
Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Fasudil hydrochloride (HA-1077) is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively.
Targets&IC50 ROCK1:0.33 μM(ki), PKC:9.3 μM(ki), PKA:1.0 μM(ki), MLCK:55 μM(ki)
Kinase Assay Cyclic AMP-dependent protein kinase activity is assayed in a reaction mixture containing, in a final volume of 0.2 mL, 50 mM Tris-HCl (pH 7.0), 10 mM magnesium acetate, 2 mM EGTA, 1 μM cyclic AMP or absence of cyclic AMP, 3.3 to 20 μM [r-32P] ATP (4×105 c.p.m.), 0.5 μg of the enzyme, 100 μg of histone H2B and compound. The mixture is incubated at 30°C for 5 min. The reaction is terminated by adding 1mL of ice-cold 20% trichloroacetic acid after adding 500 μg of bovine serum albumin as a carrier protein. The sample is centrifuged at 3000 r.p.m. for 15min, the pellet is resuspended in ice-cold 10% trichloro-acetic acid solution and the centrifugation-resuspension cycle is repeated three times. The final pellet is dissolved in 1 mL of 1 N NaOH and radioactivity is measured with a liquid scintillation counter.
Synonyms Fasudil (HA-1077) HCl, HA-1077, Fasudil HCl, HA-1077 hydrochloride, AT-877
Molecular Weight 327.83
Formula C14H18ClN3O2S
CAS No. 105628-07-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 32.8 mg/mL (100 mM)

DMSO: 24.6 mg/mL (75 mM)

TargetMolReferences and Literature

1. Ono-Saito N, et al. Pharmacol Ther, 1999, 82(2-3), 123-131. 2. Asano T, et al. J Pharmacol Exp Ther, 1987, 241(3), 1033-1040. 3. Asano T, et al. Br J Pharmacol, 1989, 98(4), 1091-1100. 4. Negoro N, et al. Biochem Biophys Res Commun, 1999, 262(1), 211-215. 5. Fukushima M, et al. Liver Int, 2005, 25(4), 829-838.

TargetMolCitations

1. Sun L, Sun L, Li X, et al. A Novel Tigecycline Adjuvant ML-7 Reverses the Susceptibility of Tigecycline-Resistant Klebsiella pneumoniae. Frontiers in cellular and infection microbiology. 2022: 1341.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Approved Drug Library TGF-beta/Smad Compound Library Anti-Cancer Drug Library Tyrosine Kinase Inhibitor Library NO PAINS Compound Library ReFRAME Related Library Epigenetics Compound Library Bioactive Compounds Library Max Reprogramming Compound Library Metabolism Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Fasudil hydrochloride 105628-07-7 Autophagy Cell Cycle/Checkpoint Chromatin/Epigenetic Cytoskeletal Signaling Membrane transporter/Ion channel Metabolism Microbiology/Virology Proteases/Proteasome Stem Cells Tyrosine Kinase/Adaptors Calcium Channel ROCK Serine/threonin kinase HIV Protease PKA PKC TWNT-4 cells TIMP-1 HA 1077 a-SMA Protein kinase A Ca channels HIV rat HSCs AT877 Rho-associated protein kinase human HSC ERK1/2 Rho-kinase inhibit Ca2+ channels Rho-associated kinase HA1077 Hydrochloride Fasudil (HA-1077) HCl p38 lysophoaphatidic acid Fasudil Ca2+ channel antagonist JNK HA 1077 Hydrochloride HA-1077 protein kinases collagen LPA HA1077 Inhibitor Fasudil HCl Fasudil Hydrochloride Protein kinase C vasodilator ROK HA-1077 hydrochloride AT 877 Human immunodeficiency virus orally active AT-877 HA-1077 Hydrochloride inhibitor

 

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