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Cinobufagin

Name: Cinobufagin
Brand: TargetMol
SKU: T3432
Price: 44 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3432Cas No. 470-37-1

Alias Cinobufagine

Cinobufagin (Cinobufagine) is a selective Na+/K+-ATPase inhibitor. The activity of Cinobufagin is same as ouabain.

Cinobufagin

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Purity: 99.97%

Catalog No. T3432Alias CinobufagineCas No. 470-37-1

Cinobufagin (Cinobufagine) is a selective Na+/K+-ATPase inhibitor. The activity of Cinobufagin is same as ouabain.

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$44	In Stock	In Stock
5 mg	$97	In Stock	In Stock
10 mg	$147	In Stock	In Stock
25 mg	$226	In Stock	In Stock
50 mg	$313	In Stock	In Stock
100 mg	$488	In Stock	In Stock
500 mg	$1,060	Inquiry	Inquiry
1 mL x 10 mM (in DMSO)	$97	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.97%

Color:White

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COA HNMR LCMS

Product Introduction

Cinobufagin AI Summary

Cinobufagin exhibits a varying degree of binding affinity against different human monoclonal antibodies, with the strongest binding observed for mAb-11E6 and mAb-5C2, as indicated by their IC50 ratios of 800.0 and 570.0, respectively. It shows weaker binding for mAb-7F2 and mAb-1B3, with IC50 ratios of 1700.0 and 98000.0, respectively. Additionally, Cinobufagin displays significant anticancer properties, showing inhibitory activity against various cell lines including PLC/PRF/5 liver carcinoma cells, HepG2, HeLa, BGC823, and Bel7402 cells with notable IC50 values. It also demonstrates strong cytotoxicity against human HL60 cells (IC50 <0.01 µg/mL) and human KB cells (IC50 = 0.21 µg/mL). Furthermore, it inhibits ELG1-dependent DNA repair with potencies ranging from 44.5 nM to 250.4 nM and inhibits Lassa and Marburg virus binding or entry into cells with potencies of 199.5 nM and 89.1 nM, respectively. Despite showing moderate inhibition against electric eel AChE and horse BChE, Cinobufagin does not exhibit significant cytotoxicity against mouse MH60 cells. Lastly, it demonstrates drug metabolism activity in various liver microsomes and recombinant CYP3A4 enzymes, indicating potential species-specific metabolism patterns..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Cinobufagin (Cinobufagine) is a selective Na+/K+-ATPase inhibitor. The activity of Cinobufagin is same as ouabain.
Synonyms	Cinobufagine

Chemical Properties

Molecular Weight	442.54
Formula	C₂₆H₃₄O₆
Cas No.	470-37-1
Smiles	CC(=O)[C@H]1[C@H]2O[C@]22[C@@H]3CC[C@@H]4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]2(C)[C@H]1c1ccc(=O)oc1
Relative Density.	1.261 g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 132.5 mg/mL (299.41 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.52 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.2597 mL	11.2984 mL	22.5968 mL	112.9841 mL
5 mM	0.4519 mL	2.2597 mL	4.5194 mL	22.5968 mL
10 mM	0.2260 mL	1.1298 mL	2.2597 mL	11.2984 mL
20 mM	0.1130 mL	0.5649 mL	1.1298 mL	5.6492 mL
50 mM	0.0452 mL	0.2260 mL	0.4519 mL	2.2597 mL
100 mM	0.0226 mL	0.1130 mL	0.2260 mL	1.1298 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc