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Chloramphenicol

Name: Chloramphenicol
Brand: TargetMol
SKU: T1205
Price: 40 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1205Cas No. 56-75-7

Alias Levomycetin, Chloromycetin, Chlornitromycin

Chloramphenicol (Chloromycetin) is a broad-spectrum antibiotic that inhibits the biosynthesis of bacterial proteins. Chloramphenicol acts primarily on the 50S subunit of the bacterial 70S ribosome and is active against peptidyltransferases, inhibiting the formation of peptide bonds.

Chloramphenicol

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Purity: 99.84%

Catalog No. T1205Alias Levomycetin, Chloromycetin, ChlornitromycinCas No. 56-75-7

Pack Size	Price	USA Warehouse	Global Warehouse
500 mg	$40	In Stock	In Stock
1 g	$48	In Stock	In Stock
5 g	$64	Inquiry	Inquiry
10 g	$93	Inquiry	Inquiry

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.84%

Appearance:Solid

Color:White

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	Chloramphenicol (Chloromycetin) is a broad-spectrum antibiotic that inhibits the biosynthesis of bacterial proteins. Chloramphenicol acts primarily on the 50S subunit of the bacterial 70S ribosome and is active against peptidyltransferases, inhibiting the formation of peptide bonds.
Targets&IC50	protozoan motility:2.95 ± 0.25 mM
In vitro	METHODS: Human lung cancer cells A549 and H1299 were treated with Chloramphenicol (1-100 µg/mL) for 3-24 h. Cell viability was detected by MTT assay. RESULTS: In the 3 h treatment group, the viability of A549 and H1299 cells at 100 µg/mL was 97.0±3.9% and 98.1±5.0%, respectively, and no cytotoxicity was observed. A significant reduction in cell viability was observed in the 24 h treatment group. [1] METHODS: Mouse T cell lines A1.1 and IE5 and human T cell lines Jurkat and Jcam were stimulated with Chloramphenicol (100 nmol/L), and the DNA content was measured by Flow cytometry. RESULTS: Chloramphenicol clearly blocked activation-induced cell death (AICD) in all four cell lines, even though these cells were activated by different stimuli. [2]
In vivo	METHODS: To investigate the potential to induce aplastic anemia, Chloramphenicol (2000 mg/kg) and Thiamphenicol (850 mg/kg) were administered by gavage to BALB/c mice once daily for 17 days. RESULTS: Administration of Chloramphenicol and Thiamphenicol at hematotoxic dose levels for 17 days in BALB/c mice was associated with the development of aplastic anemia in humans, inducing reversible anemia but not chronic bone marrow aplasia. [3]
Synonyms	Levomycetin, Chloromycetin, Chlornitromycin

Chemical Properties

Molecular Weight	323.13
Formula	C₁₁H₁₂Cl₂N₂O₅
Cas No.	56-75-7
Smiles	OCC(NC(=O)C(Cl)Cl)C(O)C1=CC=C(C=C1)[N+]([O-])=O
Relative Density.	1.547 g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight,store under nitrogen | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 1 mg/mL (3.09 mM), Sonication is recommended.

Ethanol: 60 mg/mL (185.68 mM), Sonication is recommended.

DMSO: 250 mg/mL (773.68 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (15.47 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.0947 mL	15.4736 mL	30.9473 mL	154.7365 mL

Ethanol/DMSO

	1mg	5mg	10mg	50mg
5 mM	0.6189 mL	3.0947 mL	6.1895 mL	30.9473 mL
10 mM	0.3095 mL	1.5474 mL	3.0947 mL	15.4736 mL
20 mM	0.1547 mL	0.7737 mL	1.5474 mL	7.7368 mL
50 mM	0.0619 mL	0.3095 mL	0.6189 mL	3.0947 mL
100 mM	0.0309 mL	0.1547 mL	0.3095 mL	1.5474 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc