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Brefeldin A

Catalog No. T6062 CAS 20350-15-6

Synonyms: Decumbin, 布雷非德菌素 A, Cyanein, Ascotoxin, BFA

Brefeldin A , a Penicillium brefeldianum metabolite, which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. It is also an ATPase inhibitor for protein transport in HCT 116 cells(IC50=0.2 μM), inducing Y cell differentiation and apoptosis.

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Brefeldin A, CAS 20350-15-6

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Brefeldin A , a Penicillium brefeldianum metabolite, which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. It is also an ATPase inhibitor for protein transport in HCT 116 cells(IC50=0.2 μM), inducing Y cell differentiation and apoptosis.
Targets&IC50	ATPase (HCT 116):0.2 μM
Kinase Assay	ELISA-based active site binding assay: Samples (lysed cells or tissue homogenates) are treated for 1 h at room temperature with the biotinylated active site probe PR-584 (5-15 μM). Samples are denatured by addition of SDS (0.9% final) and heating to 100 °C for 5 min. The denatured samples are transferred to a 96-well or 384-well filter plat, mixed with streptavidin-sepharose beads (2.5-5 μL packed beads/well), and incubated for 1 h at room temperature on a plate shaker. The beads are washed 5 times with 100-200 μL /well of ELISA buffer (PBS, 1% bovine serum albumin, 0.1% Tween-20) by vacuum filtration. The beads are incubated overnight at 4 °C on a plate shaker with the following antibodies recognizing the six catalytic subunits diluted into ELISA buffer: β5, β1, and β2 diluted 1:3000, LMP7 and LMP2 diluted 1:5000, and MECL-1 diluted 1:1000. The beads are washed 5 times with 100-200 μL /well of ELISA buffer and incubated with HRP-conjugated secondary antibody diluted 1:5000 in ELISA buffer and incubated 2 h at room temperature on a plate shaker. The beads are washed 5 times with 100-200 μL /well of ELISA buffer and developed for chemiluminsecence signal using the supersignal ELISA pico substrate following the manufacturer's instructions. Luminescence is measured on a plate reader and converted to ng of proteasome or μg/ml of lysate by comparison with 20S proteasome or untreated cell lysate standard curves. For proteasome inhibitor studies, active site probe binding values are expressed as the percent of binding relative to DMSO treated cells.
Cell Research	HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.(Only for Reference)

Synonyms	Decumbin, 布雷非德菌素 A, Cyanein, Ascotoxin, BFA
Molecular Weight	280.364
Formula	C16H24O4
CAS No.	20350-15-6

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

Ethanol: 2.8 mg/mL (10 mM)

DMSO: 14 mg/mL (50 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Zhu JW, et al. Bioorg Med Chem. 2000, 8(2), 455-463. 2. Ohnishi Y, et al. Br J Pharmacol. 2001, 134(1), 168-178. 3. Franco M, et al. J Biol Chem. 1996, 271(3), 1573-1578. 4. Hullin-Matsuda F, et al. J Biol Chem. 2012. 5. Wlodkowic D, et al. Leuk Res. 2007, 31(12), 1687-1700. 6. Wang J, et al. Erythroleukemia cells acquire an alternative mitophagy capability. Sci Rep. 2016 Apr 19;6:24641. 7. Zhang Y, Hu H, Liu W, et al. Amino acids and RagD potentiate mTORC1 activation in CD8+ T cells to confer antitumor immunity[J]. Journal for ImmunoTherapy of Cancer. 2021, 9(4): e002137. 8. Bai R, Li L, Liu M, et al. Paper Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis[J]. Analytical chemistry. 2018, 90(9): 5825-5832. 9. Ma X, Zou F, Yu F, et al. Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses[J]. Immunity. 2020

Citations

1. Ma X, Zou F, Yu F, et al. Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses. Immunity. 2020, 53(6): 1315-1330. e9. 2. Luo Q, Liu Q, Cheng H, et al. Nondegradable ubiquitinated ATG9A organizes Golgi integrity and dynamics upon stresses. Cell reports. 2022, 40(7): 111195. 3. Bai R, Li L, Liu M, et al. Paper Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis. Analytical chemistry. 2018, 90(9): 5825-5832. 4. Yu T, Ling Q, Xu M, et al. ORF8 protein of SARS‐CoV‐2 reduces male fertility in mice. Journal of Medical Virology. 2022

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Preclinical Compound Library Autophagy Compound Library Cancer Cell Differentiation Compound Library Anti-Cancer Metabolism Compound Library Traditional Chinese Medicine Monomer Library Antibiotics Library Inhibitor Library Macrocyclic Compound Library Natural Product Library for HTS

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Keywords

Brefeldin A 20350-15-6 DNA损伤和修复离子通道微生物学自噬 Antibiotic ATPase Autophagy CRISPR/Cas9 HSV Mitophagy Ascotoxin Cyanein Inhibitor BFA inhibit Herpes simplex virus Mitochondrial Autophagy Decumbin inhibitor

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