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6-Hydroxycoumarin

Name: 6-Hydroxycoumarin
Brand: TargetMol
SKU: T2S0357
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2S0357Cas No. 6093-68-1

Alias 6-hydroxychromen-2-one

6-Hydroxycoumarin (6-hydroxychromen-2-one) exhibits cytotoxicity against human HL60, HCT116, COLO205 cells with activity value of 35 uM, 42 uM, 37 respectively. 6-Hydroxycoumarin is a human CA9, CA12 inhibitor.

6-Hydroxycoumarin

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Purity: 98.65%

Catalog No. T2S0357Alias 6-hydroxychromen-2-oneCas No. 6093-68-1

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
100 mg	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:98.65%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC LCMS

Product Introduction

6-Hydroxycoumarin AI Summary

6-Hydroxycoumarin exhibits multifaceted bioactivity, including significant inhibition of human carbonic anhydrase (CA) enzymes with Ki values of 30.5 nM and 33.9 nM against CA9 and CA12, respectively, while showing negligible inhibition against CA1 and CA2 (Ki > 10000.0 nM). Additionally, it inhibits aldose reductase enzymes with IC50 values of 171000.0 nM for human recombinant aldose reductase 1 and 156000.0 nM for kidney aldose reductase 2, and shows selectivity over sorbitol dehydrogenase (selectivity index > 2.56). The compound demonstrates low inhibition of alpha-glucosidase (< 20% at 200 uM) and exerts weak antifungal activity against Aspergillus fumigatus (log(1/MIC) = 1.89) with high resistance in other fungal strains (MIC >= 2048.0 ug/mL). In terms of cytotoxicity, it displays activity against several human cancer cell lines (IC50 values: HCT116 = 46200 nM, COLO205 = 38700 nM, HL60 = 35000 nM), yet low cytotoxicity against PANC1 cells (LC50 > 100000 nM). It also activates Nrf2 in HSC3-ARE9 cells, indicating potential antioxidant properties (EC50 > 400000.0 nM), and exhibits antiviral activity against Influenza A virus with a 50% reduction in viral titer at 120 ug/mL. Additionally, it shows moderate inhibition of Mcl-1 (IC50 = 83690.0 nM) and significant cytotoxicity against C3PV (IC50 = 2950.0 nM) and SK-MEL-3 cells, even in the presence of Artesunic acid (IC50 = 1140.0 nM)..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	6-Hydroxycoumarin (6-hydroxychromen-2-one) exhibits cytotoxicity against human HL60, HCT116, COLO205 cells with activity value of 35 uM, 42 uM, 37 respectively. 6-Hydroxycoumarin is a human CA9, CA12 inhibitor.
Synonyms	6-hydroxychromen-2-one

Chemical Properties

Molecular Weight	162.14
Formula	C₉H₆O₃
Cas No.	6093-68-1
Smiles	Oc1ccc2oc(=O)ccc2c1
Relative Density.	1.403 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 20 mg/mL (123.35 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (30.84 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	6.1675 mL	30.8375 mL	61.6751 mL	308.3755 mL
5 mM	1.2335 mL	6.1675 mL	12.3350 mL	61.6751 mL
10 mM	0.6168 mL	3.0838 mL	6.1675 mL	30.8375 mL
20 mM	0.3084 mL	1.5419 mL	3.0838 mL	15.4188 mL
50 mM	0.1234 mL	0.6168 mL	1.2335 mL	6.1675 mL
100 mM	0.0617 mL	0.3084 mL	0.6168 mL	3.0838 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc