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Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody
😃Good
Catalog No. TMAB-00266
Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody is a Rabbit antibody targeting CA9/Carbonic Anhydrase IX. Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody
Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody
😃Good
Catalog No. TMAB-00266
Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody is a Rabbit antibody targeting CA9/Carbonic Anhydrase IX. Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody is a Rabbit antibody targeting CA9/Carbonic Anhydrase IX. Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Sample: Stomach (Mouse) Lysate at 40 μg Primary: Anti-Carbonate dehydratase IX (TMAB-00266) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 48 kDa 2. Sample: Stomach (Rat) Lysate at 40 μg Primary: Anti-Carbonate dehydratase IX (TMAB-00266) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 48/58 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CA9) Polyclonal Antibody, Unconjugated (TMAB-00266) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CA9) Polyclonal Antibody, Unconjugated (TMAB-00266) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CA9) polyclonal Antibody, Unconjugated (TMAB-00266) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. |
| Application | WB,IHC-P,IHC-Fr,ICC/IF,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:25; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | KLH conjugated synthetic peptide: human Carbonate dehydratase IX |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Associated Proteins,Vitamins / minerals,Hypoxia,Vitamins / Minerals |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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