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Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody
😃Good
Catalog No. TMAB-00266
Alias RCC, PMW1, P54/58N, MN, G250, EC 4.2.1.1, Carbonic Anhydrase IX, CA-IX, CAIX, CA9
Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody is a Rabbit antibody targeting CA9/Carbonic Anhydrase IX. Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.
Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody
Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody
😃Good
Catalog No. TMAB-00266Alias RCC, PMW1, P54/58N, MN, G250, EC 4.2.1.1, Carbonic Anhydrase IX, CA-IX, CAIX, CA9
Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody is a Rabbit antibody targeting CA9/Carbonic Anhydrase IX. Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB.
| Pack Size | Price | USA Warehouse | Global Warehouse | Quantity |
|---|---|---|---|---|
| 50 μL | $221 | 7-10 days | 7-10 days | |
| 100 μL | $372 | 7-10 days | 7-10 days | |
| 200 μL | $527 | 7-10 days | 7-10 days |
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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody is a Rabbit antibody targeting CA9/Carbonic Anhydrase IX. Anti-CA9/Carbonic Anhydrase IX Polyclonal Antibody can be used in ICC/IF, IF, IHC-Fr, IHC-P, WB. |
| Synonyms | RCC, PMW1, P54/58N, MN, G250, EC 4.2.1.1, Carbonic Anhydrase IX, CA-IX, CAIX, CA9 |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse) |
| Verified Activity | 1. Sample: Stomach (Mouse) Lysate at 40 μg Primary: Anti-Carbonate dehydratase IX (TMAB-00266) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 48 kDa 2. Sample: Stomach (Rat) Lysate at 40 μg Primary: Anti-Carbonate dehydratase IX (TMAB-00266) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 48/58 kDa 3. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CA9) Polyclonal Antibody, Unconjugated (TMAB-00266) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded (human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation with (CA9) Polyclonal Antibody, Unconjugated (TMAB-00266) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (CA9) polyclonal Antibody, Unconjugated (TMAB-00266) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.      |
| Application | |
| Recommended Dose | ICC/IF=1:50-200; IF=1:100-500; IHC-Fr=1:100-500; IHC-P=1:100-500; WB=1:500-2000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus. |
| Tissue Specificity | Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1 mg/mL |
| Research Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated synthetic peptide: human Carbonate dehydratase IX |
| Antigen Species | Human |
| Gene Name | CA9 |
| Gene ID | |
| Protein Name | Carbonic anhydrase 9 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Associated Proteins,Vitamins / minerals,Hypoxia,Vitamins / Minerals |
| Function | Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. |
| Molecular Weight | Theoretical: 46 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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