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2,5-Di-tert-butylhydroquinone

Name: 2,5-Di-tert-butylhydroquinone
Brand: TargetMol
SKU: T7540
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7540Cas No. 88-58-4

Alias BHQ

2,5-Di-tert-butylhydroquinone (BHQ) is an effective and selective endoplasmic reticulum Ca2+-ATPase inhibitor.

2,5-Di-tert-butylhydroquinone

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Purity: 97.11%

Catalog No. T7540Alias BHQCas No. 88-58-4

2,5-Di-tert-butylhydroquinone (BHQ) is an effective and selective endoplasmic reticulum Ca2+-ATPase inhibitor.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
10 g	$29	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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Expected start date of shipmentUSA Warehouse [1-2 days] Global Warehouse [5-7 days]

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Purity:97.11%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	2,5-Di-tert-butylhydroquinone (BHQ) is an effective and selective endoplasmic reticulum Ca2+-ATPase inhibitor.
Targets&IC50	Ca2+-ATPase:66.7 μM, COX-2:14.1 μM, 5-LOX:1.8 μM
In vitro	2,5-Di-tert-butylhydroquinone shifted the voltage dependence of the steady-state inactivation curve to more negative potentials by 7 mV in the mid-potential of the curve, without affecting the activation curve as well as the time course of I(Ca(L)) inactivation.Preincubation of the cells either with 10 microM cyclopiazonic acid, a SERCA inhibitor, or with 3 mM diethyldithiocarbamate, an inhibitor of intracellular superoxide dismutase (SOD), did not modify 2,5-Di-tert-butylhydroquinone inhibition of I(Ca(L)).On the contrary, this effect was no longer evident when SOD (250 u ml(-1)) was added to the perfusion medium.Either in the presence or in the absence of cells, 2,5-Di-tert-butylhydroquinone gave rise to superoxide anion formation, which was markedly inhibited by the addition of SOD.At micromolar concentrations, 2,5-Di-tert-butylhydroquinone inhibits vascular I(Ca(L)) by giving rise to the formation of superoxide anion which in turn impairs the channel function.
Synonyms	BHQ

Chemical Properties

Molecular Weight	222.32
Formula	C₁₄H₂₂O₂
Cas No.	88-58-4
Smiles	CC(C)(C)C1=CC(O)=C(C=C1O)C(C)(C)C
Relative Density.	1.07 g/cm3
Color	White
Appearance	Solid

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (224.9 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (9 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.4980 mL	22.4901 mL	44.9802 mL	224.9010 mL
5 mM	0.8996 mL	4.4980 mL	8.9960 mL	44.9802 mL
10 mM	0.4498 mL	2.2490 mL	4.4980 mL	22.4901 mL
20 mM	0.2249 mL	1.1245 mL	2.2490 mL	11.2451 mL
50 mM	0.0900 mL	0.4498 mL	0.8996 mL	4.4980 mL
100 mM	0.0450 mL	0.2249 mL	0.4498 mL	2.2490 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc