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(S)-10-Hydroxycamptothecin

Name: (S)-10-Hydroxycamptothecin
Brand: TargetMol
SKU: T2764
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2764Cas No. 19685-09-7

Alias 10-Hydroxycamptothecin, 10-HCPT

(S)-10-Hydroxycamptothecin (10-HCPT) is a DNA topoisomerase I inhibitor used as a clinical therapeutic agent against hepatoma.

(S)-10-Hydroxycamptothecin

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Purity: 99.81%

Catalog No. T2764Alias 10-Hydroxycamptothecin, 10-HCPTCas No. 19685-09-7

(S)-10-Hydroxycamptothecin (10-HCPT) is a DNA topoisomerase I inhibitor used as a clinical therapeutic agent against hepatoma.

Pack Size	Price	USA Warehouse	Global Warehouse
200 mg	$51	In Stock	In Stock
500 mg	$108	In Stock	In Stock
1 g	$189	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.81%

Color:Yellow

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	(S)-10-Hydroxycamptothecin (10-HCPT) is a DNA topoisomerase I inhibitor used as a clinical therapeutic agent against hepatoma.
In vitro	In the CAM model, 10-Hydroxycamptothecin (25 nM) inhibits angiogenesis in a concentration-dependent manner. In mice carrying Colo 205 xenografts, oral administration of 10-Hydroxycamptothecin (2.5-7.5 mg/kg) every two days significantly suppresses tumor cell growth.
In vivo	(S)-10-Hydroxycamptothecin inhibits cell growth in BT-20 cells (IC50=34.3 nM) and MDA-231 cells (IC50=7.27 nM), demonstrating its efficacy. It also induces the formation of cleavable complexes mediated by human topoisomerase I in pBR322 plasmid DNA (EC50=0.35 μM). Additionally, in human microvascular endothelial cells (HMEC), (S)-10-Hydroxycamptothecin dose-dependently inhibits cell growth (IC50=0.31 μM), significantly hampers HMEC migration (IC50=0.63 μM), and impedes angiogenesis (IC50=0.96 μM).
Cell Research	Cells are exposed to various concentrations of 10-Hydroxycamptothecin for 72 hours. Cell growth is monitored by the standard MTT assay.(Only for Reference)
Synonyms	10-Hydroxycamptothecin, 10-HCPT

Chemical Properties

Molecular Weight	364.35
Formula	C₂₀H₁₆N₂O₅
Cas No.	19685-09-7
Smiles	O=C1N2C(C=3C(C2)=CC=4C(N3)=CC=C(O)C4)=CC5=C1COC(=O)[C@@]5(CC)O
Relative Density.	1.60 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 26 mg/mL (71.36 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7446 mL	13.7231 mL	27.4461 mL	137.2307 mL
5 mM	0.5489 mL	2.7446 mL	5.4892 mL	27.4461 mL
10 mM	0.2745 mL	1.3723 mL	2.7446 mL	13.7231 mL
20 mM	0.1372 mL	0.6862 mL	1.3723 mL	6.8615 mL
50 mM	0.0549 mL	0.2745 mL	0.5489 mL	2.7446 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc