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Tyrphostin 8

Name: Tyrphostin 8
Brand: TargetMol
SKU: T34973
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T34973Cas No. 3785-90-8

Alias 4-Hydroxybenzylidenemalononitrile

Tyrphostin 8(4-Hydroxybenzylidenemalononitrile) is a potent GTPase inhibitor that inhibits EGFR kinase with an IC50 of 560 μM.Tyrphostin 8 inhibits protein serine/threonine calmodulin phosphatases (IC50=21 μM), enhances transferrin receptor-mediated transcytosis in Caco-2 cells, and increases the hypoglycemic effect of oral insulin-transferrin. hypoglycemic effect.

Tyrphostin 8

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Purity: 97%

Catalog No. T34973Alias 4-HydroxybenzylidenemalononitrileCas No. 3785-90-8

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$31	-	In Stock
10 mg	$45	-	In Stock
25 mg	$73	-	In Stock
50 mg	$106	-	In Stock
100 mg	$157	-	In Stock
500 mg	$389	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:97%

Appearance:Solid

Color:Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Tyrphostin 8(4-Hydroxybenzylidenemalononitrile) is a potent GTPase inhibitor that inhibits EGFR kinase with an IC50 of 560 μM.Tyrphostin 8 inhibits protein serine/threonine calmodulin phosphatases (IC50=21 μM), enhances transferrin receptor-mediated transcytosis in Caco-2 cells, and increases the hypoglycemic effect of oral insulin-transferrin. hypoglycemic effect.
Targets&IC50	EGFR:560 μM (IC50), Calcineurin phosphatase:21 μM (IC50)
In vitro	Pretreated for 20 minutes at concentrations ranging from 10 to 100 μM, Tyrphostin 8 blocks Carbachol-initiated PKCδ tyrosine phosphorylation and ERK1/2 activation in parotid acinar cells[1]. Additionally, at concentrations of 10-100 μM, Tyrphostin 8 induces a rapid and substantial increase in the basal O2 consumption of parotid acinar cells[1]. Furthermore, at a concentration of 100 μM, Tyrphostin 8 reduces parotid ATP content by approximately 90%[1]. In another context, Tyrphostin 8 enhances the apical-to-basolateral transport of insulin-transferrin conjugate by promoting transferrin receptor-mediated transcytosis in filter-grown Caco-2 cell monolayers[2].
In vivo	In Streptozotocin-induced diabetic rats, Tyrphostin 8 enhances the glucose-lowering effect of insulin-transferrin[2].
Synonyms	4-Hydroxybenzylidenemalononitrile

Chemical Properties

Molecular Weight	170.17
Formula	C₁₀H₆N₂O
Cas No.	3785-90-8
Smiles	OC1=CC=C(C=C(C#N)C#N)C=C1
Relative Density.	1.31g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (293.82 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	5.8765 mL	29.3824 mL	58.7648 mL	293.8238 mL
5 mM	1.1753 mL	5.8765 mL	11.7530 mL	58.7648 mL
10 mM	0.5876 mL	2.9382 mL	5.8765 mL	29.3824 mL
20 mM	0.2938 mL	1.4691 mL	2.9382 mL	14.6912 mL
50 mM	0.1175 mL	0.5876 mL	1.1753 mL	5.8765 mL
100 mM	0.0588 mL	0.2938 mL	0.5876 mL	2.9382 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc