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N-Acetyl-5-hydroxytryptamine

Name: N-Acetyl-5-hydroxytryptamine
Brand: TargetMol
SKU: T1354
Price: 32 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1354Cas No. 1210-83-9

Alias O-Demethylmelatonin, Normelatonin, N-Acetylserotonin

N-Acetyl-5-hydroxytryptamine (O-Demethylmelatonin) , also known as Normelatonin, is the immediate precursor of melatonin. It is produced from serotonin by the enzyme aralkylamine N-acetyltransferase and is converted to melatonin by acetylserotonin O-methyltransferase. It can potently activate TrkB receptor.

N-Acetyl-5-hydroxytryptamine

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Purity: 99.91%

Catalog No. T1354Alias O-Demethylmelatonin, Normelatonin, N-AcetylserotoninCas No. 1210-83-9

Pack Size	Price	USA Warehouse	Global Warehouse
10 mg	$32	In Stock	In Stock
25 mg	$48	In Stock	In Stock
50 mg	$67	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.91%

Color:White to Yellow

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COA HPLC HNMR

Product Introduction

Bioactivity

Description	N-Acetyl-5-hydroxytryptamine (O-Demethylmelatonin) , also known as Normelatonin, is the immediate precursor of melatonin. It is produced from serotonin by the enzyme aralkylamine N-acetyltransferase and is converted to melatonin by acetylserotonin O-methyltransferase. It can potently activate TrkB receptor.
In vitro	N-acetylserotonin activates TrkB receptor in a circadian rhythm. N-Acetyl-5-hydroxytryptamine swiftly activates TrkB in a circadian manner and exhibits antidepressant effect in a TrkB-dependent manner. N-Acetyl-5-hydroxytryptamine rapidly activates TrkB, but not TrkA or TrkC, in a neurotrophin- and MT3 receptor-independent manner[1].
In vivo	To investigate the potential of N-Acetyl-5-hydroxytryptamine (NAS) to activate TrkB in vivo, researchers utilized TrkB F616A knockin mice, which have been demonstrated to exhibit selective inhibition of TrkB F616A activation by 1NMPP1, a derivative of the kinase inhibitor PP1, resulting in TrkB-null phenotypes. The study aimed to determine if NAS could replicate the effects of BDNF. Cortical neurons from TrkB F616A knockin mice were prepared for this purpose. Supporting previous findings, the study showed that NAS and BDNF-induced TrkB phosphorylation were specifically diminished by 1NMPP1, unlike by K252a, while serotonin or melatonin had no impact. These results suggest that NAS significantly induces tyrosine phosphorylation and activation of both wild-type TrkB and TrkB F616A[1].
Synonyms	O-Demethylmelatonin, Normelatonin, N-Acetylserotonin

Chemical Properties

Molecular Weight	218.25
Formula	C₁₂H₁₄N₂O₂
Cas No.	1210-83-9
Smiles	CC(=O)NCCc1c[nH]c2ccc(O)cc12
Relative Density.	1.268 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 131.25 mg/mL (601.37 mM), Sonication is recommended.

Ethanol: 10 mg/mL (45.82 mM), Sonication is recommended.

H2O: 2 mg/mL (9.16 mM), Sonication and heating are recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (9.16 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.5819 mL	22.9095 mL	45.8190 mL	229.0951 mL
5 mM	0.9164 mL	4.5819 mL	9.1638 mL	45.8190 mL

Ethanol/DMSO

	1mg	5mg	10mg	50mg
10 mM	0.4582 mL	2.2910 mL	4.5819 mL	22.9095 mL
20 mM	0.2291 mL	1.1455 mL	2.2910 mL	11.4548 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0916 mL	0.4582 mL	0.9164 mL	4.5819 mL
100 mM	0.0458 mL	0.2291 mL	0.4582 mL	2.2910 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc