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Colcemid

Name: Colcemid
Brand: TargetMol
SKU: T19720
Price: 279 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T19720Cas No. 477-30-5

Alias NSC-3096, Kolchicin, Demecolcine, C-12669, C12669, C 12669

Colcemid (NSC-3096) is a microtubule polymerization inhibitor, with the main target being Tubulin and an IC50 of 2.4 μM. Colcemid can induce apoptosis and can be used in the research of tumors and embryo cloning.

Colcemid

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Purity: 99.83%

Catalog No. T19720Alias NSC-3096, Kolchicin, Demecolcine, C-12669, C12669, C 12669Cas No. 477-30-5

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$279	In Stock	In Stock
5 mg	$686	In Stock	In Stock
10 mg	$975	In Stock	In Stock
25 mg	$1,430	In Stock	In Stock
50 mg	$1,880	In Stock	In Stock
100 mg	$2,800	-	In Stock
1 mL x 10 mM (in DMSO)	$695	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.83%

Appearance:Solid

Color:White to Yellow

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COA HNMR LCMS

Product Introduction

Colcemid AI Summary

Colcemid demonstrates significant bioactivity and pharmacological properties across various assays. It inhibits the polymerization of purified bovine brain tubulin with an IC50 of 2400.0 nM and displaces [3H]colchicine from tubulin with an IC50 of 3580.0 nM. The compound shows potent cytotoxicity against multiple human cancer cell lines, including MCF7, H460, and SF-268, with EC50 values of 600.0 nM, 690.0 nM, and 6200.0 nM, respectively. Additionally, it exhibits cytotoxicity against KB cells with an ED50 of less than 0.25 µg/ml. Colcemid also causes cell cycle arrest at the G2/M phase in human MCF7 and HeLa cells, indicative of its potential as an antimitotic agent. In vivo, Colcemid shows effectiveness against lymphocytic leukemia P388 in mice, with a potency of 10.8 µM/kg and toxicity values leading to an LD50 of 236.7 µM/kg after a single intramuscular injection. It also exhibits acute toxicity in mice with an LD50 of 237.0 mg/kg. In terms of lipophilicity, it has a partition coefficient (logP) value of 1.53 and logD value of 1.37 at pH 7.4. Moreover, the compound has no significant antimicrobial activity against Micrococcus luteus, Mycobacterium smegmatis, Saccharomyces cerevisiae, and Aspergillus niger, with MIC values greater than 500 µg/ml. Finally, Colcemid shows activation of human and rat pregnane X receptor (PXR), with competitive binding affinity to the human PXR ligand-binding domain (LBD), suggesting moderate potency in these assays. It also affects mRNA translation, nuclear factor kappa B (NF-kB) activation, and induces an irreversible mitotic block in human A2780S cells..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Colcemid (NSC-3096) is a microtubule polymerization inhibitor, with the main target being Tubulin and an IC50 of 2.4 μM. Colcemid can induce apoptosis and can be used in the research of tumors and embryo cloning.
Targets&IC50	SF-268 cells:6.2 μM (EC50), HepG2 cells:0.09 μM (EC50), tubulin:2.4 μM (EC50), NCI-H460 cells:0.69 μM (EC50), MCF-7 cells:0.6 μM (EC50)
Synonyms	NSC-3096, Kolchicin, Demecolcine, C-12669, C12669, C 12669

Chemical Properties

Molecular Weight	371.43
Formula	C₂₁H₂₅NO₅
Cas No.	477-30-5
Smiles	COC1=C2C(C([C@H](CCC2=CC(OC)=C1OC)NC)=C3)=CC=C(OC)C3=O
Relative Density.	1.21g/cm3

Storage & Solubility Information

Storage

keep away from moisture,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 55 mg/mL (148.08 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.38 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.6923 mL	13.4615 mL	26.9230 mL	134.6149 mL
5 mM	0.5385 mL	2.6923 mL	5.3846 mL	26.9230 mL
10 mM	0.2692 mL	1.3461 mL	2.6923 mL	13.4615 mL
20 mM	0.1346 mL	0.6731 mL	1.3461 mL	6.7307 mL
50 mM	0.0538 mL	0.2692 mL	0.5385 mL	2.6923 mL
100 mM	0.0269 mL	0.1346 mL	0.2692 mL	1.3461 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc