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CDDO-Im

Name: CDDO-Im
Brand: TargetMol
SKU: TQ0120
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. TQ0120Cas No. 443104-02-7

Alias TP-235, RTA-403, CDDO-Imidazolide

CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).

CDDO-Im

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Purity: 99.61%

Catalog No. TQ0120Alias TP-235, RTA-403, CDDO-ImidazolideCas No. 443104-02-7

CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$31	In Stock	In Stock
5 mg	$72	In Stock	In Stock
10 mg	$113	In Stock	In Stock
25 mg	$222	In Stock	In Stock
50 mg	$355	In Stock	In Stock
100 mg	$538	In Stock	In Stock
500 mg	$1,150	-	In Stock
1 mL x 10 mM (in DMSO)	$86	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.61%

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).
Targets&IC50	PPARγ:344 nM (Ki), PPARα:232 nM (Ki)
In vitro	CDDO-Im effectively suppresses the proliferation of human leukemia and breast cancer cell lines (IC50 approximately 10-30 nM). In U937 leukemia cells, it induces monocytic differentiation, evidenced by increased CD11b and CD36 expression [1]. CDDO-Im treatment causes G2/M-phase cell cycle arrest and apoptosis in triple-negative breast cancer cell lines SUM159 and MDA-MB-231, significantly inhibiting CD24−/EpCAM+ cells in SUM159 tumorspheres. It also decreases sphere-forming efficiency and size in both primary and secondary cultures [2]. Moreover, CDDO-Im elevates Nrf2 protein levels, which increases the expression of antioxidant and detoxification genes regulated by Nrf2 [3].
In vivo	CDDO-Im exhibits strong inhibition of de novo inducible nitric oxide synthase (iNOS) expression in primary mouse macrophages. Additionally, it effectively suppresses the proliferation of B16 murine melanoma and L1210 murine leukemia cells in vivo. Administering 10 nM (5.4 μg) of CDDO-Im significantly impedes the induction of iNOS by IFN-γ, while a dose as low as 1 nmol (0.54 μg) of CDDO-Im shows partial effectiveness [1].
Cell Research	CDDO-Im is dissolved in DMSO. SUM159 and MDA-MB-231 cells are seeded into each well of 96-well plates (1,000 cell/well) and treated the next day with vehicle control or CDDO-Im (1, 10, 50, 100 and 200 nM) for given incubation time. The absorbance is measured with a spectrophotometer to determine the cell proliferation rate [2].
Animal Research	Mice are injected i.p. with thioglycollate, and the resulting resident peritoneal macrophages are activated 3 days later with an i.p. injection of IFN-γ. CDDO and CDDO-Im are injected i.p. 30 min after IFN-γ. Macrophages are harvested 10 h later, cultured for 12 h, and then assayed for expression of iNOS protein and production of nitric oxide (NO) [1].
Synonyms	TP-235, RTA-403, CDDO-Imidazolide

Chemical Properties

Molecular Weight	541.72
Formula	C₃₄H₄₃N₃O₃
Cas No.	443104-02-7
Smiles	[H][C@@]12CC(C)(C)CC[C@@]1(CC[C@]1(C)[C@]2([H])C(=O)C=C2[C@@]1(C)CC[C@@]1([H])C(C)(C)C(=O)C(=C[C@]21C)C#N)C(=O)n1ccnc1
Relative Density.	1.23 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 40 mg/mL (73.84 mM), Sonication is recommended.

H2O: Insoluble

In Vivo Formulation

10% DMSO+90% Corn Oil: 2 mg/mL (3.69 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8460 mL	9.2299 mL	18.4597 mL	92.2986 mL
5 mM	0.3692 mL	1.8460 mL	3.6919 mL	18.4597 mL
10 mM	0.1846 mL	0.9230 mL	1.8460 mL	9.2299 mL
20 mM	0.0923 mL	0.4615 mL	0.9230 mL	4.6149 mL
50 mM	0.0369 mL	0.1846 mL	0.3692 mL	1.8460 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc