Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF,FCM

Reactivity: Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Horse,Rabbit)

IgG

Human,Mouse,Rat (predicted:Chicken,Pig,Cow,Horse,Rabbit)

1. Sample:
Lane1: Heart (Mouse) Lysate at 30 μg
Lane2: Liver (Mouse) Cell Lysate at 30 μg
Primary: Anti-PPAR alpha (TMAB-01565) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit Igg (secondary antibody) at 1: 5000dilution;
Predicted band size: 51 kDa
Observed band size: 51 kDa
2. Rat splenocytes stained with Anti-PPAR alpha Polyclonal Antibody, PE-CY5 Conjugated (TMAB-01565-PE-Cy5) at 1:50.
3. Sample: Heart (Mouse) Lysate at 40 μg
Primary: Anti-PPAP alpha (TMAB-01565) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
4. Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-PPAR alpha antibody (TMAB-01565)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PPAR alpha) Polyclonal Antibody, Unconjugated (TMAB-01565) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Blank control: Jurkat.
Primary Antibody (green line): Rabbit Anti-PPAR alpha antibody (TMAB-01565)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
7. Sample: Heart (Mouse) Lysate at 40 μg Heart (Rat) Lysate at 40 μg Liver (Mouse) Lysate at 40 μg Urinary bladder (Mouse) Lysate at 40 μg Kidney (Mouse) Lysate at 40 μg
Primary: Anti-PPAR alpha (TMAB-01565) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52/19 kDa
Observed band size: 52 kDa

WB,IHC-P,IHC-Fr,IF,FCM

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1μg /test

Polyclonal

KLH conjugated synthetic peptide: human PPAR alpha

Human

5465

Metabolism of lipids and lipoproteins,Response to hypoxia,Nuclear hormone receptors,Metabolism,Zinc Finger,Fatty acids,Lipid metabolism,Mitochondrial transcription,Hypoxia,Mitochondrial Biogenesis,Fatty acid oxidation,Obesity