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Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody
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Catalog No. TMAB-01462
Alias p-NFE2L2 (Ser40), p-NFE2L2 (S40), Phospho-NFE2L2 (S40), NFE2L2 (p-Ser40), NFE2L2 (p-S40)
Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFE2L2 (Ser40). Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody can be used in WB,ICC/IF.
Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody
Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody
😃Good
Catalog No. TMAB-01462Alias p-NFE2L2 (Ser40), p-NFE2L2 (S40), Phospho-NFE2L2 (S40), NFE2L2 (p-Ser40), NFE2L2 (p-S40)
Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFE2L2 (Ser40). Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody can be used in WB,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFE2L2 (Ser40). Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody can be used in WB,ICC/IF. |
| Alias | p-NFE2L2 (Ser40), p-NFE2L2 (S40), Phospho-NFE2L2 (S40), NFE2L2 (p-Ser40), NFE2L2 (p-S40) |
| Ig Type | IgG |
| Reactivity | Human,Mouse (predicted:Rat,Chicken,Dog,Cow) |
| Verified Activity | 1. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) polyclonal Antibody, Unconjugated (TMAB-01462) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus. 2. Sample: Lane 1: Hela (Human) Cell Lysate at 30 μg Lane 2: HepG2 (Human) Cell Lysate at 30 μg Lane 3: MCF-7 (Human) Cell Lysate at 30 μg Lane 4: Jurkat (Human) Cell Lysate at 30 μg Lane 5: B16 (Human) Cell Lysate at 30 μg Lane 6: A549 (Human) Cell Lysate at 30 μg Primary: Anti-phospho-Nrf2 (Ser40) (TMAB-01462) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kDa Observed band size: 135 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; ICC/IF: 1:100 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which regulates the expression of many detoxification and antioxidant enzymes. Nrf2 can potentially play a significant role in adaptive responses to oxidative stress. Nrf2 belongs to the Cap N Collar (CNC-bZIP) subfamily of basic /leucine zipper (bZIP) transcription factors. |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human Nrf2 around the phosphorylation site of Ser40 |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Cardiac metabolism,Oxidative Stress,ChIP antibodies,Other factors,Mitochondrial transcription,Mitochondrial Biogenesis,Oxidative stress |
| Molecular Weight | Theoretical: 68 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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