Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody is a Rabbit antibody targeting Phospho-NFE2L2 (Ser40). Anti-Phospho-NFE2L2 (Ser40) Polyclonal Antibody can be used in ICC/IF, WB.

IgG

Human (predicted:Mouse,Rat,Chicken,Dog,Cow)

1. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (phospho-Nrf2 (Ser40)) polyclonal Antibody, Unconjugated (TMAB-01462) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Sample:
Lane 1: Hela (Human) Cell Lysate at 30 μg
Lane 2: HepG2 (Human) Cell Lysate at 30 μg
Lane 3: MCF-7 (Human) Cell Lysate at 30 μg
Lane 4: Jurkat (Human) Cell Lysate at 30 μg
Lane 5: B16 (Human) Cell Lysate at 30 μg
Lane 6: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-Nrf2 (Ser40) (TMAB-01462) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 135 kDa

ICC/IF=1:100-500; WB=1:500-2000

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Nrf2 around the phosphorylation site of Ser40

Human

NFE2L2

Nuclear factor erythroid 2-related factor 2

Cardiac metabolism,Oxidative Stress,ChIP antibodies,Other factors,Mitochondrial transcription,Mitochondrial Biogenesis,Oxidative stress

Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.